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Do Culinary Mushrooms Have Fibrinolytic Activities?

Sharjahan Mohamed Ali, Yee-Shin Tan, Jegadeesh Raman, Hariprasath Lakshmanan, Tau-Chuan Ling, Chia-Wei Phan, Vikineswary Sabaratnam

Abstract

Recently, many culinary mushrooms have become attractive sources of biologically but non-pharmacological activities including immune modulating activities. In this study, the fibrinolytic activities of freeze-dried basidiocarps of ten edible mushrooms were determined using a qualitative fibrin plate assay and a quantitative enzyme assay using spectrophotometric method. Further, the crude extracts of all the ten mushrooms were subjected to an aqueous two-phase system (ATPS) to recover the fibrinolytic enzymes. The crude extracts and ATPS extracts of Lentinula edodes showed the highest fibrinolytic activity of 48.06 U/mg and 54.28 U/mg, respectively assessed via the Folin-spectrophotometric method. The recovery of fibrinolytic enzymes from L. edodes was the highest and the fibrinolytic enzymes were further analysed through gel electrophoresis study. A 50 kDa sized fibrinolytic enzyme from L. edodes was revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Biomed Rev 2017; 28:91-99.

 

Keywords: edible mushroom, fibrinolytic enzyme, Lentinula edodes, aqueous two-phase system


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DOI: http://dx.doi.org/10.14748/bmr.v28.4454

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About The Authors

Sharjahan Mohamed Ali
University of Malaya, Kuala Lumpur
Malaysia

Mushroom Research Centre

Yee-Shin Tan
University of Malaya, Kuala Lumpur
Malaysia

Mushroom Research Centre

Institute Biological Sciences, Faculty of Science

Jegadeesh Raman
University of Malaya, Kuala Lumpur Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Republic of Korea
Malaysia

Mushroom Research Centre

Hariprasath Lakshmanan
University of Malaya, Kuala Lumpur Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India
Malaysia

Mushroom Research Centre

Tau-Chuan Ling
University of Malaya, Kuala Lumpur
Malaysia

Mushroom Research Centre

Institute Biological Sciences, Faculty of Science

Chia-Wei Phan
University of Malaya, Kuala Lumpur
Malaysia

Mushroom Research Centre

Department of Pharmacy, Faculty of Medicine

Vikineswary Sabaratnam
University of Malaya, Kuala Lumpur
Malaysia

Mushroom Research Centre

Institute Biological Sciences, Faculty of Science

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