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Antibodies recognizing globular domain of C1q - current view on the association between lupus nephritis manifestation and anti-gC1q autoantibodies

Maria Atanasova Radanova, Vishnya Stoyanova, Kamelia Bratoeva, Vasil Vasilev, Valentin Ikonomov, Diana Ivanova

Abstract

Introduction: Lupus nephritis (LN) is a serious complication of the systemic lupus erythematosus (SLE). Anti-C1q antibodies correlate with the occurrence and high clinical activity of LN, especially proliferative LN. The first reported anti-C1q antibodies recognized autoepitopes within collagen-like region (CLR) of C1q. Recently we have found autoantibodies against globular C1q domain (gC1q antibodies) in LN patients.

The aim of the present study was to evaluate the potential pathological consequences of the presence of anti-gC1q antibodies in LN.

Material and Methods: The recombinant globular head region of the three chains of C1q -A, -B and -C were expressed in E. coli BL21 and purified. Anti-C1q, anti-gC1q autoantibodies, complement proteins - C1q, C4, C3 and IgG-, IgM-CICs levels were screened by ELISA in 53 sera from LN patients. Sera from 196 normal controls served as controls.

Results: We found that patients positive for anti-B-gC1q antibodies presented with significantly lower serum C4 levels than patients positive for anti-A and anti-C-gC1q antibodies (p = 0.014) and with significantly lower levels of C3 than patients positive for anti-A and anti-C-gC1q antibodies and patients without anti-C1q antibodies (p = 0.005; p = 0.018). Significant correlations to IgG CICs were detected for anti-C1q (r = 0.371, p = 0.001) and anti-B-gC1q antibodies (r = 0.431, p = 0.003).

Conclusions: These findings suggest that the binding of anti-B-gC1q autoantibodies with C1q may possibly trigger mechanical stress and induce a structural change within the CLR domain of C1q, compatible with C1r-C1s complement activation in the fluid phase.


Keywords

anti-C1q autoantibodies; anti-gC1q autoantibodies; lupus nephritis

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DOI: http://dx.doi.org/10.14748/ssm.v1i1.1381

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About The Authors

Maria Atanasova Radanova
Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna
Bulgaria

Vishnya Stoyanova
Department of Chemistry, Biochemistry, Physiology, and Pathophysiology, Sofia University
Bulgaria

Kamelia Bratoeva
Department of Physiology and Pathophysiology, Medical University of Varna
Bulgaria

Vasil Vasilev
Clinics of Nephrology, University Hospital - `Tzaritza Ioanna - ISUL`, Medical University - Sofia

Valentin Ikonomov
Clinics of Nephrology, Acute and Peritoneal Dialysis, Apheresis and Transplantation, University Hospital - `St. Marina`, Medical University of Varna

Diana Ivanova
Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

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