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Histological study on the postnatal alterations in the rat kidney

Stancho Stanchev, Alexandar Iliev, Lina Malinova, Boycho Landzhov, Dimka Hinova-Palova

Abstract

INTRODUCTION: The aging kidney is characterized by numerous morphological changes in the nephrons and interstitium which are usually associated with failure of renal function. The aim of the present study is to describe and compare histological age-related changes in the kidney in three age groups of rats.

MATERIALS AND METHODS: Male Wistar rats aged 2, 4 and 6 months (n=3; per age group) were used in the present study. Alterations in kidney morphology were observed by light microscopic analysis using PAS reaction and Mallory`s trichrome staining.

RESULTS: We observed normal histological structure of the kidney in 2-month-old rats. We noted that renal corpuscles were relatively well preserved and proximal and distal tubules were visible as well-defined structures. No pathological changes in blood vessels and interstitial fibrosis were observed. As age advanced (4 and 6 months) we described periglomerular and periarteriolar sclerosis, wrinkling and thickening of glomerular and tubular basement membranes; interstitial fibrosis was generally expressed.

CONCLUSION: Renal aging is a multifactorial process characterized by an increased number of sclerotic glomeruli, tubular atrophy and interstitial fibrosis and is associated with declining renal function.


Keywords

kidney, age-related changes, rat

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References

Bolignano D, Mattace-Raso F, Sijbrands EJ, Zoccali C. The aging kidney revisited: a systematic review. Ageing Res Rev. 2014; 14:65-80.

Pannarale G, Carbone R, Del Mastro G, Gallo C, Gattullo V, Natalicchio L, et al. The aging kidney: structural changes. J Nephrol. 2010; 23(Suppl. 15):S37-40.

Weinstein JR, Anderson S. The aging kidney: physiological changes. Adv Chronic Kidney Dis. 2010; 17(4):302-7.

Yabuki A, Yoneshige S, Tanaka S, Tsujio M, Mitani S, Yamato O. Age-related histological changes in kidneys of Brown Norway rat. J Vet Med Sci. 2014; 76(2):277-80

Baylis C, Corman B. The aging kidney: insights from experimental studies. J Am Soc Nephrol. 1998; 9(4):699-709.

Smith SM, Hoy WE, Cobb L. Low incidence of glomerulosclerosis in normal kidneys. Arch Pathol Lab Med. 1989;113(11):1253-5.

Martin JE, Sheaff MT. Renal ageing. J Pathol. 2007; 211(2):198-205.

Ferder LF, Inserra F, Basso N. Effects of the reninangiotensin system blockade in the aging kidney. Exp Gerontol. 2003; 38:237-44.

Long DA, Mu W, Price KL, Johnson RJ. Blood vessels and the aging kidney. Nephron Exp Nephrol. 2005; 101(3):e95-9.

Zou XJ, Saxena R, Liu Z, Vaziri ND, Silva FG. Renal senescence in 2008: progress and challenges. Int Urol Nephrol. 2008; 40:823-39.

Silva FG. The aging kidney: a review - part I. Int Urol Nephrol. 2005; 37:185-205.

Moritz AR, Oldt ME: Arteriolar sclerosis in hypertensive and nonhypertensive individuals. Am J Pathol. 1937; 13:679-86.

Olson JL. Hyaline arteriolosclerosis: New meaning for an old lesion Kidney International, Vol. 63 (2003), p. 1162-1163

Fioretto P, Mauer M. Histopathology of diabetic nephropathy. Semin Nephrol. 2007; 27(2):195-207.

Bohle A, Wehrmann M, Greschniok A, Junghans R. Renal morphology in essential hypertension: analysis of 1177 unselected cases. Kidney Int Suppl. 1998; 67:S205-6.

Bolton WK, Sturgill BC. Spontaneous glomerular sclerosis in aging Sprague-Dawley rats. II. Ultrastructural studies. Am J Pathol. 1980; 98(2):339-56.

Yumura W, Sugino N, Nagasawa R, Kubo S, Hirokawa K, Maruyama N. Age-associated changes in the renal glomeruli of mice. Exp Gerontol. 1989; 24(3):237-49.

Nishida E, Yamanouchi J, Ogata S, Itagaki S, Doi K. Age-related histochemical and ultrastructural changes in renal glomerular mesangium of APA hamsters. Exp Anim. 1996; 45(4):339-45.

Hirokawa K. Characterisation of age-associated kidney disease in Wistar rats. Mech Ageing Dev. 1975; 4:301-16.

Efstratiadis G, Divani M, Katsioulis E, Vergoulas G. Renal fibrosis. Hippokratia. 2009;13(4):224-9.

Hoy WE, Douglas-Denton RN, Hughson MD, Cass A, Johnson K, Bertram JF. A stereological study of the glomerular number and volume: preliminary findings in a multiracial study of kidneys at autopsy. Kidney Int. 2003; 63:S31-7.

Manalich R, Reyes L, Herrera M, Melendi C, Fundora I. Relationship between weight and the number and size of renal glomeruli in humans: a histomorphometric study. Kidney Int. 2000; 58:770-3.

Anderson S, Brenner BM. Effect of aging on the renal glomerulus. Am J Med. 1986; 80:436-42.

Mudler WJ, Hillen HFP. Renal function and renal disease in the elderly: part I. Eur J Intern Med. 2001; 12:86-97.

Abrass CK, Adcox MJ, Raugi GJ. Aging associated changes in renal extracellular matrix. Am J Pathol. 1995; 146:742-52.

Inserra F, Romano LA, de Cavanagh EM, Ercole L, Ferder LF, Gomez RA. Renal interstitial sclerosis in aging: effects of enalapril and nifedipine. J Am Soc Nephrol. 1996; 7:676-80.

Takazakura E, Sawabu N, Handa A, Takada A, Shinoda A, Takeuchi J. Intrarenal vascular changes with age and disease. Kidney Int. 1972; 2:224-30.

Bolton WK, Sturgill BC. Spontaneous glomerular sclerosis in aging- Sprague-Dawley rats. Am J Pathol. 1980; 98:339-50.

Lindeman RD. Is the decline in renal function with normal aging inevitable? Geriatr Nephrol Urol. 1998; 8:7-9.




DOI: http://dx.doi.org/10.14748/ssm.v49i1.1936

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About The Authors

Stancho Stanchev
Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria
Bulgaria

Alexandar Iliev
Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria
Bulgaria

Lina Malinova
Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria
Bulgaria

Boycho Landzhov
Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria
Bulgaria

Dimka Hinova-Palova
Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria
Bulgaria

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