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Purification of Enolase from Human Brain Tissue

B. Galunska, D. Zanev, H. Divanyan

Abstract

Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) were purified using a small number of methods performed in the following order: DEAE-cellulose chromatography, diafiltration, preparative isoelectric focusing and gel-filtration. Pure isoenzymes were isolated in quantities, which would allow us to produce antisera against each form of the enzyme.


Keywords

Neuron-Specific Enolase; Non-Neuronal Enolase; Purification of Enolase; DEAE-Chromatography; Preparative Isoelectring Focusing; Electrophoresis

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References

Цанев, Д., X. Диванян. Схема за ултрафилтрация. Изобретение, per. № 74294/27.III. 1986 г.

Aoki, Т., Т. Tanaka, Н. Watabe. Purification and characterization of ?-enolase from various mammals.- Chem. Pharm. Bull., 40, 1992, 1236-1239.

Chekhonin, V., I. Gurina, S. Portnaya, et al. Monoclonal anti-NSE-antibodies: purification, characterization, and immunoenzyme analysis.- Vopr. Med. Khim, 48, 2002, 477-484.

Fletcher, L., С. Rider, С. Taylor. Enolase isoenzymes. III. Chromatographic and immunological characteristics of rat brain enolase.- Biochim. Biophys. Acta, 452, 1976, 245-252.

Gorsich, S., V. Barrows, J. Halbert, et al. Purification and properties of gamma-enolase from pig brain.-J. Protein Chem., 18, 1999, 103-115.

Gulick, A., K. Hubbard, A. Gerlt, I. Rayment. Evolution of enzymatic activities in the enolase superfamily: crystallographic and mutagenesis studies of the reaction catalyzed by D-glutharate

dehydratase from Escherichia coli.- Biochemistry, 39, 2000, 4590-4602.

Harding, M. Neuron-specific enolase (NSE) in small cell lung cancer: a tumor marker of prognostic significance.- Br. J. Cancer, 61, 1990, 605-607.

Kustrzeba-Wojcicka, I., M. Golczak. Enolase from Candida albicans - purification and characterization.- Biochem. Mol. Biol., 126, 2000, 109-120.

Marangos, P. In: Multiple Forms of Enzymes. D. Scheuch, R. Hashen, F. Hofmann, eds. Berlin, VEB Verlag Volk und Gesundheit, 1982, 214-219.

Marangos, P., A. Parma, F. Godwin. Functional properties of neuronal and glial isoenzymes of brain enolase.-J. A'eurochem., 31, 1978, 727-732.

Marangos, P. A. Zis, R. Clark, et al. Neuronal, non-neuronal and hybrid forms of enolase in brain: structural, immunological and functional comparisons.- Brain Res., 150, 1978, 117-133.

Marangos, P., C. Zomzely-Neurath, D. Luk, C. York. Isolation and characterization of the nervous system-specific protein 14-3-2 from rat brain.- J. Biol. Chem., 250, 1975, 1884-1891.

Sartophor-Methoden. Isoelektrische Fokussierung (IEF) mit dem Sartophor-System und Sartorius

Servalyt-Precotes.- SM/IEF, 5, 1983.

Sartophor. Bestimmung der PGM-Untergruppen durch isoelektrische Fokussierung.- SM/PGM, 5a, 1984.

Stoscheck, C. Quantitation of protein.- Meth. Enzymol., 1982, 1990, 50-53.

Susor, W., W. Rutter. Some distinctive properties of pyruvate kinase purified from rat liver.- Biochem. Biophys. Res. Commun., 30, 1968, 14-20.

Suzuki, F., Y. Umeda, K. Kato. Rat brain enolase isoenzymes.- J. Biochem., 87, 1980, 1587-1594.

Vermuyten, K., A. Lowenthal, D. Larcher. Detection of neuron-specific enolase concentrations

in cerebrospinal fluid from patients with neurological disorders by means of a sensitive enzyme immunoassay.- Clin. Chim. Acta, 187, 1990, 69-78.

Wirnsberger, G. Diagnostic immunohistochemistry of neuroblast tumors.- Am. J. Surg. Pathol.. 16, 1992, 49-57.

Zelzer, P. Serum neuron-specific enolase in children with neuroblastoma.- Cancer, 57, 1986, 1230-1234.




DOI: http://dx.doi.org/10.14748/ssm.v35i0.1946

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About The Authors

B. Galunska

Bulgaria

B. Galunska, Dept, of Preclinical and Clinical Pharmacology and Biochemistry, Prof. P. Stoyanov Medical University of Varna. 55 Marin Drinov St, BG-9002 Varna, BULGARIA
E-mail: pharmacol@asclep.muvar.acadbg

D. Zanev

H. Divanyan

Bulgaria

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