Scientific Online Resource System

Scripta Scientifica Medica

INTRATHYROID LYMPHOCYTE SUBSETS IN PATIENTS WITH AUTOIMMUNE THYROID DISEASE

K. Hristozov, L. Koeva, S. Balev, Ts. Odzhakova

Abstract

Thyroid gland is an easily approachable for direct examination by aspiration biopsy organ. This fact gives a possibility for serial examination of liable disturbances in the immune regulation, connected with autoimmune thyroid disease and their response to the relevant therapy. Twenty-six patients with Basedow's disease (BD) and 12 patients with Hashimoto's thyroiditis (HT) - 34 women and 4 men (mean age 47,3 years) were studied. For determination of superficial markers of intrathyroidal mononuclear cells (ITMC) were used monoclonal antibodies for identification of total T-lymphocytes (CD3+), Т-helper/ inducer cells (CD4+), T-suppressor/cytotoxic eels (CD8+), B-lymphocytes (CD 19+), Nklymphocytes (CD16+56+), activated Т-lymphocytes (CD3+/HLA-DR+), interleukin-2 receptors (CD 25+), and the adhesion molecule ICAM-1 (CD54+). In the patients with BD the main lymphocyte subset was CD3+ with increased number of activated T-lymphocytes and CD4/CD8 ratio (2,22) due to the decreased number of T-suppressor/cytotoxic cells. After the thyreostatic therapy there was a normalization of B-lymphocytes, activated Tlymphocytes and reduction of CD4/CD8 ratio, due to the increased number of T-suppressor/ cytotoxic cells. In patients with HT and hypothyroidism the main cell population consisted of CD3+cells and increased number of CD16+56+ cells as the CD4/CD8 ratio was decreased (0,98). The presence of increased percentage ofIL-2 receptors and of adhesion molecules ICAM-1 in these patients suggests a more pronounced activity of the autoimmune process.


Keywords

Thyroid gland diseases; Т-lymphocytes; B-lymphocytes; ICAM-1 adhaesion molecules; intrathyroidal mononuclear cells

Full Text


References

Davies, T. F. Israel J. Med. 5c/., 1994, 2-11.

Eguchi, К., N. Matsuoka, S. Nagataki. Clin. Endocrinol. Metab., 9, 1995, 243-254.

MacKenzie, W. A . , A. W. Schwartz, E . W. Fridman, et a l . J. Clin. Endocrinol. Metab. 64, 1987, 818-824.

Nagataki, S., K. Eguchi. Autoimmunity, 13, 1992, 27-34.

Ohashi, H., T. Okugawa, M. Itoh. Acta Endocrinol, 125, 1991,502-509.

Paschke, R., M. Vogg, R. Kristoferitsch, et al. J. Clin. Endocrinol. Metab., 80, 1995, No 8, 2470-2474.

Waifish, P., K . Tseng: Autoimmunity, 13, 1992, 35-42.

Yoshikawa, N., T . Morita, E . Resetkova, et al. J.Endocrinol. Invest., 15, 1992, 609-617.




DOI: http://dx.doi.org/10.14748/ssm.v30i0.2854

Refbacks

Article Tools
Email this article (Login required)
About The Authors

K. Hristozov

L. Koeva

S. Balev

Ts. Odzhakova

Font Size


|