Scientific Online Resource System

Scripta Scientifica Medica

FLOWCYTOMETRIC STUDIES OF CELL IMMUNITY IN PATIENTS WITH URAEMIA ON PERIODIC HAEMODIALYSIS AND AFTER RENAL TRANSPLANTATION

S. Staikova, S. Balev, K. Nenov, K. Kisyova, D. Nenov

Abstract

The course of a series of kidney diseases is characterized by impaired immunity additionally altered during haemodialysis and after renal transplantation. The study covered 50 patients divided into three groups: 16 patients with chronic renal failure (CRF), 23 ones on periodic haemodialysis (PHD), and 11 after renal transplantation. Flowcytometric immunophenotyping by means of FACS-analysis using monoclonal antibodies was carried out. The investigation aimed at revealing the changes in the cell immunity, activation of surface markers and adhesion molecules in these patients. Statistically significant changes in the expression of 1С AM-1 adhesion molecules were established as followed: CD54+/ CD4+ = 24,8 ± 11,6 (in CRF) against 14,9 ±8,1 (in PHD) atp < 0,05 and CD54+/CD8+ = 17 ± 6,9 (in CRF) against 10,3 ± 4,8 (in PHD) at p < 0,05. A conclusion was drawn that immune response damage in PHD patients resulted mainly from the lowered expression of the adhesion molecules of the ICAM-1 immunoglobulin gene superfamily.


Keywords

Chronic renal failure; haemodialysis; flow cytometry, immunophenotyping; ICAM-1 adhesion molecules

Full Text


References

Наумова, Е. В: Клинична имунология. Под ред. Б. Божков, М. Огнянов.София, Знание, 1997, 393-400.

Огнянов, М., И. Алтънкова. В: Имунология на вътрешните болести. София, Медицина и физкултура, 1990, 9-44.

Cibrik, D. М., J. R. S ed or. In: Primer on Kidney Diseases. 2. ed. A . Greenberg, ed. San Diego, etc., Academic Press, 1998,141-149.

Matsumoto, J. , T . Shinzato, I . Takai, et a L Nephrol., Dial. Transplant., 13, 1998, No 11, 2861-2866.

Vanholder, R . , R. De Smet, V. Jacobs, et a l . Nephrol., Dial. Transplant., 9, 1994, No 9, 1271-1278.




DOI: http://dx.doi.org/10.14748/ssm.v30i0.2860

Refbacks

Article Tools
Email this article (Login required)
About The Authors

S. Staikova

S. Balev

K. Nenov

K. Kisyova

D. Nenov

Font Size


|