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Scripta Scientifica Medica

Immunohistochemistry of synaptic proteins for synaptic exocytosis

Wladimir Ovtscharoff, Manfred Gratzl, Boycho Landzhov, Lina Malinova, Dimka Hinova-Palova, Minko Minkov, Adrian Paloff, Lazar Jelev

Abstract

The synaptic proteins synaptobrevin/VAMP, SNAP-25, Syntaxin1, NSF and α-SNAP were revealed by means of immunocytochemistry. Materials from the cerebral cortex of adult, newborn and postnatal rats (P6 and P11) were used. Immunostaining for synaptobrevin/VAMP was mainly around the synaptic vesicles, whereas the immunolabeling for SNAP-25 and syntaxin1 was revealed in most cases on the cytoplasmic surface of the presynaptic membrane and to a lesser extend - on synaptic vesicles. Immunostaning for NSF and α-SNAP was found out not only on the axoplasm of axonal endings and varicosities, but also in perikarya and dendrites. In the cerebral cortex of newborn rats, a small number of immunopositive presynaptic parts could be observed. The number of these immunolabeled structures increases evidently with increasing age of rats.

Scripta Scientifica Medica 2013; 45(1): 29-34.


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DOI: http://dx.doi.org/10.14748/ssm.v45i1.334

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About The Authors

Wladimir Ovtscharoff
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology, Medical Faculty

Manfred Gratzl
Technical University of Munich
Germany

Anatomical Institute

Boycho Landzhov
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Lina Malinova
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Dimka Hinova-Palova
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Minko Minkov
Medical University of Varna
Bulgaria

Department of Anatomy, Histology and Embryology

Adrian Paloff
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Lazar Jelev
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

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