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Scripta Scientifica Medica

Comparative electron microscopic and immunohistochemical study of stromal cells in giant cell tumor of bone

Georgi P. Georgiev, Boycho Landzhov, Svetoslav A. Slavchev, Pavel Rashev, Todor Todorov, Lina Malinova, Luben Stokov, Wladimir Ovtscharoff

Abstract

Giant cell tumor of bone is an osseous neoplasm that is histologically benign but clinically shows local aggression and high rate of recurrence. The histogenesis of this lesion remains unclear. The histological appearance does not predict the clinical outcome and there are still many unanswered questions with regard to both its treatment and prognosis. In order to further clarify this lesion, we examined ultrastructurally and immunohistochemically the tumor mononuclear cells in ten patients operated on in our hospital for matrix metalloproteinase-9. Positive reaction was detected in the spindle-like stromal cells of giant cell tumor of bone and these cells had the ultrastructural characteristics of fibroblastic cells. The other mononuclear cells did not express matrix metalloproteinase-9 and showed ultrastuctural characteristics of macrophage-like cells. The positive reaction for matrix metalloproteinase-9 in all patients clearly shows that this protease may play a key role in the pathophysiology of giant cell tumor of bone.

Keywords

giant cell tumor of bone; ultrastructural study; matrix metalloproteinase-9

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References

Aparisi, T., B. Arborgh, J. L. Ericsson. Giant cell tumor of bone: detailed fine structural analysis of different cell components. - Virchows Arch. A . Pathol. Anat. Histol., 376, 1977, No 4, 273-298.

Cowan, R. W., G. Singh. Giant cell tumor of bone: A basic science perspective. - Bone, 52, 2013, No 1, 238-246.

Georgiev, G. P., L. Stokov. Giant cell tumor of bone. - Bulg. J. Orthop. Trauma, 49, 2012, No 1, 34-39.

Georgiev, G. P., B. Landzhov, S. Slavchev, P. Rashev, L. Stokov, W. Ovtscharoff. Localization of matrix metalloproteinase-2 in giant cell tumor of Fig. 2. Electron micrograph of a mononuclear spindleshaped cell (x 8700) Fig. 3. Electron micrograph of a mononuclear polygonal cell (x 9000) 22 Scripta Scientifica Medica, vol. 45, 2013, suppl. 1, pp. 19-22

Copyright © Medical University of Varna Comparative electron microscopic and immunohistochemical study of stromal cells in giant cell tumor of bone bone. - Compt. rend. Acad. bulg. Sci., 65, 2012, No 9, 1285-1288.

Georgiev, G. P., H. Georgiev, B. Landzhov. Ultrastructural study of giant cell tumor of bone.

-Rp./Orthop. Rheumatol., 2012, No 2-3, 13-15 (in Bulgarian).

Georgiev, H., B. Matev, N. Dimitrov, P. Georgiev. Osteoclastoma of metacarpal bones. - Acta Med. Bulg., 38, 2011; No 1, 3-7.

Ghert, M., N. Simunovic, R. W. Cowan, N. Colterjohn, G. Singh. Properties of the stromal

cell in giant cell tumor of bone. - Clin. Orthop. Relat. Res., 459, 2007, 8-13.

Kim, Y., S. Nizami, H. Goto, F. Y. Lee. Modern interpretation of giant cell tumor of bone:

predominantly osteoclastogenic stromal tumor. - Clin. Orthop. Surg., 4, 2012, No 2, 107-116.

Matev, B., H. Georgiev, G. P. Georgiev. Giant cell tumor of the fourth metacarpal: case report and literature review. - J. Radiother. Med. Oncol., 18, 2012, No 2, 73-77.

Rabinovich, A., I. W. Mak, R. W. Cowan, R. E. Turcotte, N. Colterjohn, G. Singh, M. Ghert.

Matrix metalloproteinase activity in the stromal cell of giant cell tumor of bone. - Open Bone J., 1, 2009, 46-52.

Shingleton, W. D., D. J. Hodges, P. Brick, T. E. Cawston. Collagenase: a key enzyme in collagen turnover. - Biochem. Cell Biol., 74, 1996, No 6, 759-775.

Steiner, G. C., L. Ghosh, H. D. Dorfman. Ultrastructure of giant cell tumors of bone. -

Hum Pathol., 3, 1972, No 4, 569-586.

Szendröi, M. Giant-cell tumour of bone. - J. Bone Joint Surg. Br., 86, 2004, No 1, 5-12.

Turcotte, R. E., J. S. Wunder, M. H. Isler, R. S. Bell, N. Schachar, B. A. Masri, G. Moreau, A. M. Davis. Giant cell tumor of long bone: a Canadian Sarcoma Group study. - Clin. Orthop. Relat. Res., 397, 2002, 248-258.

Viguet-Carrin, S., P. Garnero, P. D. Delmas. The role of collagen in bone strength. - Osteoporos. Int., 17, 2006; No 3, 319-336.

Wülling, M., C. Engels, N. Jesse, M. Werner, G. Delling, E. Kaiser. The nature of giant cell tumor of bone. - J. Cancer Res. Clin. Oncol., 127, 2001, No 8, 467-474.

Zheng, M. H., P. Robbins, J. Xu, L. Huang, D. J. Wood, J. M. Papadimitriou. The histogenesis of giant cell tumour of bone: a model of interaction between neoplastic cells and osteoclasts. - Histol. Histopathol., 16, 2001, No 1, 297-307.




DOI: http://dx.doi.org/10.14748/ssm.v45i0.832

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About The Authors

Georgi P. Georgiev
Medical University of Sofia
Bulgaria

University Hospital of Orthopaedics `Prof. B. Boychev"

Boycho Landzhov
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Svetoslav A. Slavchev
Medical University of Sofia
Bulgaria

University Hospital of Orthopaedics `Prof. B. Boychev"

Pavel Rashev
Medical University of Sofia
Bulgaria

Department of Immunobiology of Reproduction

Todor Todorov
Medical University of Sofia
Bulgaria

Department of Pathology

Lina Malinova
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

Luben Stokov
Medical University of Sofia
Bulgaria

University Hospital of Orthopaedics `Prof. B. Boychev"

Wladimir Ovtscharoff
Medical University of Sofia
Bulgaria

Department of Anatomy, Histology and Embryology

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