Scientific Online Resource System

Scripta Scientifica Medica

Immunohistochemical study on distribution of cannabinoid cb1 receptors in the rat`s prefrontal cortex after cold stress procedure

Boycho Landzhov, Elena Dzhambazova, Lina Malinova, Lawrence Edelstein, Anastasia Bozhilova-Pastirova, Dimka Hinova-Palova, Minko Minkov, Adrian Paloff, Wladimir Ovtscharoff

Abstract

The prefrontal cortex (PFC), which mediates the emotional coping response to different stressful paradigms, is composed of distinct parts depends on stimulus involved physical or psychological stress. It also plays a role in a number of neurological conditions. It`s known that neuroendocrine control of homeostatic and reproductive functions including stress response and energy metabolism is fulfils by important signaling molecules as endogenous cannabinoids. The aim of the present study was to examine the effects of cold stress on distribution of CB1- receptors in PFC of rats. Immunohistochemical procedure for CB1-receptors was performed in adult male Wistar rats. The data were entered in the computer program, recorded automatically, calculated and compared by Student`s t-test. We found CB1-immunoreaction in axons and dendrites as well as in cell bodies where they presented as puncta on somata. The cells bodies were comprised of several distinct shapes: pyramidal, oval, fusiform and multipolar. Numerous fine-beaded fibers and puncta were seen on a handful of pyramidal large-sized neurons and many puncta were observed around the oval-shaped small- and medium-sized neurons.The PFC in cold stress rats demonstrated around 18% higher density of CB1-receptors compared with controls. In conclusion our results showed that cold stress exposure increased distribution of CB1-receptors in PFC of rats. These experimental data suggest that endocannabinoid system in this brain area may play an important role in the continuity of homeostasis in cold stress.

Keywords

CB1-immunoreactive neurons; cold stress; prefrontal cortex; rats

Full Text


References

Bambico, F. R., N. Katz, G. Debonnel, G. Gobbi.

(2007) Cannabinoids elicit antidepressant-like

behavior and activate serotonergic neurons

through the medial prefrontal cortex.- J.

Neurosci., 27, 2008, 11700-11711.

Bechara, A., A. R. Damasio, H. Damasio, S. W.

Anderson. Insensitivity to future consequences

following damage to human prefrontal cortex.

-Cognition, 50, 1994, No 1-3, 7-15.

Bechara, A., H. Damasio, D. Tranel, A. R.

Damasio. Deciding advantageously before

knowing the advantageous strategy. -Science,

, 1997; No 5304, 1293-5.

Bhatia, N., P. P. Maiti, A. Choudhary, A. Tuli, D.

Masih, M. U. Khan, T. Ara, A.S. Jaggi. Animal

models in the study of stress: A review. -NSHM

Journal of Pharmacy and Healthcare Management,

, 2011, 42-50.

Bozhilova-Pastirova, A., A. Bocheva, E.

Dzambazova, B. Landzhov, L. Malinova,

W. Ovtscharoff. Distribution patterns of

CB1 receptors in rat`s cerebral cortex after

immobilization stress. Immunocytochemical

study. -Scripta Scientifica Medica, 43, 2011 No 2,

p. 114.

Deshmukh, S., K. Onozuka, K. J. Bender, V.

A. Bender, B. Lutz, K. Mackie, D. E. Feldman.

Postnatal development of cannabinoid receptor

type 1 expression in rodent somatosensory cortex.

-Neurosci., 145, 2007, No 1, 279-87.

Dzambazova, E., B. Landzhov, A. Bocheva, A.

Bozhilova-Pastirova. Effects of kyotorphin on

NADPH-d reactive neurons in rat`s Cerebral

cortex after acute immobilization stress. -Compt.

Rend. Acad. Bulg. Sci., 64, 2011. No 12, 1779-1784.

Edelstein, L., F. Denaro, J. S. Stamm, B. Landzhov,

L. Malinova, D. Hinova-Palova, A. Paloff, A.

Bozhilova-Pastirova, E. Dzhambazova, A.

Bocheva, W. Ovtscharoff. Distribution of CB1

receptors in the claustrum of rats undergoing

acute stress: An immunohistochemical study.

-Neuroscience Meeting Planner. Washington, DC:

Society for Neuroscience, 2011. Online, 734.12/

AAA18.

Häring, M., S. Guggenhuber, B. Lutz.

Neuronal populations mediating the effects of

endocannabinoids on stress and emotionality.

-Neurosci., 204, 2012, 145-58.

Jankord R, J. P. Herman. Limbic regulation of

hypothalamopituitary-adrenocortical function

during acute and chronic stress. -Ann. N. Y. Acad.

Sci., 2008, No 1148, 64-73.

Keay, K. A., R. Bandler. Parallel circuits

mediating distinct emotional coping reactions to

different types of stress. -Neurosci. Biobehav. Rev.,

, 2001, No 7-8, 669-78.

Marsicano, G., R. Kuner. Anatomical

distribution of receptors, ligands and enzymes

in the brain and the spinal cord: circuitries and

neurochemistry. In: Cannabinoids and the brain

(Kofalvi A, ed), Springer, 2008, 161-202.

Ongur, D., J. L. Price. The organization of

networks within the orbital and medial prefrontal

cortex of rats, monkeys and humans.-Cereb.

Cortex, 10, 2000, 206-219.

Pacák, K., M. Palkovits. Stressor specificity of

central neuroendocrine responses: implications

for stress-related disorders. -Endocr. Rev., 22,

, 502-548,.

Pencheva, N., K. Grancharska, A. Bocheva,

E. Dzambazova, B. Landzhov, L. Malinova,

A Bozhilova-Pastirova, W. Ovtscharoff.

Immunocytochemical study of CB1 receptors in

rat`s prefrontal cortex after immobilization stress.

-Compt. Rend. Bulg. Acad. Sci., 65, 2012, No 7,

-1008.

Petrovich, G. D., P. C. Holland, M. Gallagher.

Amygdalar and prefrontal pathways to the lateral

hypothalamus are activated by a learned cue

that stimulates eating. -J. Neurosci., 25, 2005,

-8302.

Pijlman, F.T., G. Wolterink, J. M. Van Ree.

Physical and emotional stress have differential

effects on preference for saccharine and open field

behaviour in rats. -Behav. Brain. Res., 139, 2003,

No 1-2, 131-138.

Price, J. L., S. T. Carmichael, W. C. Drevets.

Networks related to the orbital and medial

prefrontal cortex; a substrate for emotional

behavior. -Prog. Brain. Res., 107, 1996, 523-36.

Tanji, J., E. Hoshi. Role of the lateral prefrontal

cortex in executive behavioral control. -Physiol.

Rev., 88, 2008, 37-57.




DOI: http://dx.doi.org/10.14748/ssm.v45i0.834

Refbacks

Article Tools
Email this article (Login required)
About The Authors

Boycho Landzhov
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Elena Dzhambazova
Department of physiology and pathophysiology
Bulgaria

University of Sofia

Lina Malinova
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Lawrence Edelstein
Medimark corp., San Diego, CA
United States

Anastasia Bozhilova-Pastirova
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Dimka Hinova-Palova
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Minko Minkov
Medical university of Varna
Bulgaria

Department of anatomy, histology and embryology

Adrian Paloff
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Wladimir Ovtscharoff
Medical university of Sofia
Bulgaria

Department of anatomy, histology and embryology

Font Size


|