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Scripta Scientifica Medica

Effect of frozen storage on fat soluble vitamins content in fish fillets

Diana A. Dobreva, Albena Merdzhanova, Mona Stancheva

Abstract

Fat-soluble vitamins content (all-trans-retinol, alpha-tocopherol and cholecalciferol) in edible tissue of Bluefish (Pomatomus saltatrix), a typical Black sea pelagic fish, and in Rainbow trout (Oncorhynchus mykiss), a typical farmed freshwater fish, were determined and compared on raw state and after frozen storage. The sample preparation procedure includes saponification and consequent extraction of fat-soluble vitamins with n-hexane.

The extract was dried under nitrogen flow and redissolved in methanol. HPLC analysis of methanolic samples was performed on ODS2 Hypersil (250x4,6, 5um) column with a mobile phase of methanol:water = 97:3. The quantification of fat-soluble vitamins was by the method of standard addition. Retinol and cholecalciferol were monitored by UV detection and alpha-tocopherol was detected by fluorescence.

The retinol and cholecalciferol contents in fresh edible tissue of Black sea Bluefish (38.5±2.4 μg.100g-1ww and 11.2±1.2 μg.100g-1ww, respectively) were close to values in the freshwater fish Rainbow trout (58.9±2.6 μg.100g-1ww and 14.9±1.1 μg.100g-1ww, respectively). Alpha-tocopherol content was several fold higher in Rainbow trout (1648.9±68.8 μg.100g-1ww) than in Black sea Bluefish (427.1±37.1 μg.100g-1ww).

Long period of storage affected mostly retinol and alpha-tocopherol contents in two fish species. While cholecalciferol content remained almost unchanged.


Keywords

Retinol; Alpha-Tocopherol; Cholecalciferol; Frozen storage; Fish fillet

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DOI: http://dx.doi.org/10.14748/ssm.v45i0.926

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About The Authors

Diana A. Dobreva
Medical University of Varna
Bulgaria

Department of Chemistry

Albena Merdzhanova
Medical University of Varna
Bulgaria

Department of Chemistry

Mona Stancheva
Medical University of Varna
Bulgaria

Department of Chemistry

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