Scientific Online Resource System

Scripta Scientifica Medica

Effect of aronia melanocarpa fruit juice on alcohol-induced depressive-like behavior in rats

Stefka Valcheva-Kuzmanova, Miroslav Eftimov, Petko Denev, Maria Krachanova, Anna Belcheva

Abstract

PURPOSE: The present study investigated the effects of Aronia melanocarpa fruit juice (AMFJ) on behavior and biochemical indices of liver toxicity and oxidative stress in female Wistar rats subchronically treated with ethanol.

MATERIAL AND METHODS: Ethanol was applied for 14 days at a daily dose of 8 g/kg divided in two equaloral gavages. AMFJ was applied as a pretreatment 1 hour before each ethanol administration at doses of 2,5 ml/kg, 5 ml/kg and 10 ml/kg. Effects on behavior were investigated using the open field test (OFT) for locomotor activity, elevated plus maze test (EPM) for anxiety and forced swim test (FST) for depression. The biochemical markers were: liver enzymes for liver toxicity and thiobarbituric acid reactive substances (TBARS) for oxidative stress.

RESULTS: Ethanol induced a significant reduction in locomotor activity in the OFT and EPM. The FST showed that ethanol induced a depressive-like behavior. These effects were not accompanied by liver toxicity. AMFJ did not significantly affect the reduced by ethanol central nervous excitability demonstrated in the OFT and EPM. It showed an anti-depressive effect as evidenced by the reduction of immobility time in the FST. This effect is not likely to be due to the pronounced antioxidant activity of AMFJ as ethanol did not induce oxidative stress estimated by the TBARS.

CONCLUSION: In this model of ethanol-induced changes in rat behavior, AMFJ showed an anti-depressant like effect which might be due to the polyphenolic ingredients of the juice.


Keywords

Aronia melanocarpa; alcohol; behavior; anti-depressant; female rats

Full Text


References

Hasin, D. S., F. S. Stinson, E. Ogburn, B. F. Grant. Prevalence, correlates, disability, and comorbidity of DSM-IV alcohol abuse and dependence in the United States: results from the National Epidemiologic Survey on Alcohol and Related Conditions.- Arch. Gen. Psychiatry, 64, 2007, No 7, 830-842.

Paulke, A., M. Nöldner, M. Schubert-Zsilavecz, M. Wurglics. St. John’s wort flavonoids and their metabolites show antidepressant activity and accumulate in brain after mutiple oral dose.- Pharmazie, 63, 2008, No 4, 296-302.

Singleton, V., J. Rossi. Colorimetry of total phenolic with phosphomolibdiphosphotungstic acid reagents.- Am. J. Enol. Viticult., 16, 1965, 144-158.

Howell, A. B., J. D. Reed, C. G. Krueger, R. Winterbottom, D. G. Cunningham, M. Leahy. A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity.- Phytochemistry, 66, 2005, No 18, 2281-2291.

Ou, B., M. Hampsch-Woodill, R. L. Prior. Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescence probe.- J. Agric. Food Chem., 49, 2001, No 10, 4619-4626.

Ou, B., M. Hampsch-Woodill, J. Flanagan, E. K. Deemer, R. L. Prior, D. J. Huang. Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe.- J. Agric. Food Chem., 50, 2002, No 10, 2772-2777.

Gould, T. D., T. D. Dao, C. E. Kavacsics. The open field test.- In: Mood and anxiety related pheno LLC, 2009, 1-2.

Pellow, S., P. Chopin, S. E. File, M. Briley. Validation of open:closed arm entries in an elevated plus-maze as a measure of anxiety in the rat.- J. Neurosci. Methods, 14, 1985, No 3, 149-167.

Porsolt, R. D. Animal model of depression.- Biomedicine, 30, 1979, No 3, 139-140.

Ohkawa, H., Ν. Ohishi, Κ. Yagi. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction.- Anal. Biochem., 95, 1979, 351-358.

File, S. E., C. Fernandes. Dizocilpine prevents the development of tolerance to the sedative effects of diazepam in rats.- Pharmacol. Biochem. Behav., 1994, 47, No 4, 823-826.

Rygula, R., N. Abumaria, G. Flügge, E. Fuchs, E. Rüther, U. Havemann-Reinecke. Anhedonia and motivational deficits in rats: Impact of chronic social stress.- Behav. Brain Res., 162, 2005, No 1, 127-134.

Katz, R. J., K. A. Roth, B. J. Carroll. Acute and chronic stress effects on open field activity in the rat: implications for a model of depression.- Neurosci. Biobehav. Rev., 5, 1981, No 2, 247-251.

Getachew, B., S. R. Hauser, R. E. Taylor, Y. Tizabi. Alcohol-induced depressive-like behavior is associated with cortical norepinephrine reduction.- Pharmacol. Biochem. Behav., 96, 2010, No 4, 395-401.

Takeda, H., M. Tsuji, M. Inazu, T. Egashira, T. Matsumiya. Rosmarinic acid and caffeic acid produce antidepressive-like effect in the forced swimming test in mice.- Eur. J. Pharmacol., 449, 2002, No 3, 261-267.

Machado, D. G., L. E. B. Bettio, M. P. Cunha, A. R. Santos, M. G. Pizzolatti, I. M. Brighente, et al. Antidepressant-like effect of rutin isolated from the ethanolic extract from Schinus molle L. in mice: Evidence for the involvement of the serotonergic and noradrenergic systems.- Eur. J. Pharmacol., 587, 2008, No 1-3, 163-168.




DOI: http://dx.doi.org/10.14748/ssm.v45i0.933

Refbacks

Article Tools
Email this article (Login required)
About The Authors

Stefka Valcheva-Kuzmanova
Medical University of Varna
Bulgaria

Department of Preclinical and Clinical Pharmacology

Miroslav Eftimov
Medical University of Varna
Bulgaria

Department of Preclinical and Clinical Pharmacology

Petko Denev

Bulgaria

Institute of Oranic Chemistry with Centre of Phytochemistry - BAS, Laboratory of Biologically Active Substances, Plovdiv

Maria Krachanova

Bulgaria

Institute of Oranic Chemistry with Centre of Phytochemistry - BAS, Laboratory of Biologically Active Substances, Plovdiv

Anna Belcheva
Medical University of Varna
Bulgaria

Department of Preclinical and Clinical Pharmacology

Font Size


|