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Accuracy of central and peripheral cornea thickness: a comparison of two clinically available instruments

Teodora Marinova, Vesela Ivancheva, Valeri Sheherov, Christina Grupcheva

Abstract

Introduction : To compare the thickness measurements of central and peripheral cornea in healthy human subjects without eye symptomology, using laser scanning confocal microscopy (LSCM) and anterior segment OCT (AS-OCT), two technologies based on optical principle.

Materials and Methods : The study was based on evaluating repeatability and comparability of two different methods for measuring the corneal thickness in central and four peripheral corneal zones. Data were analyzed in two age groups, group A, 22±2 years (n= 27) and group B, 60±11 years (n=29). Fifty six subjects were clinically examined to observe corneal structure and confirm that there are no pathological changes. Each participant was then examined by AS-OCT and following topical anaesthesia by LSCM.

Results : The mean values of average central corneal thickness by anterior segment optical coherence tomography (AS-OCT) and Laser-scanning confocal microscopy (LSCM) in the group of younger individuals were 553.33±12.1 μm and 573.33±57.22 μm, respectively for the central cornea and 734.33±22.1 μm and 773.83±72.22 μm for corneal periphery. The group of aged participants presented results of CCT measured by AS-OCT and LSCM as follow: 561.12±19.1 μm and 593.93±60.72 μm, respectively for the central cornea and 742.33±30.1 μm and 789.31±78.92 μm for corneal periphery. LSCM demonstrated lower repeatability.

Conclusion : The study demonstrated that AS-OCT is better method for measurement of corneal thickness of subjects regardless their age. Furthermore the method is less invasive and provides thickness measurement of 70-80% of the corneal surface. The two methods should be combined to achieve comprehensive structural and morphological analysis of the human cornea in health and disease.


Keywords

cornea; thickness; confocal microscopy; optical coherence tomography

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DOI: http://dx.doi.org/10.14748/ssm.v45i0.935

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About The Authors

Teodora Marinova
Medical University of Varna
Bulgaria

Department of Ophthalmology and Visual Science

Vesela Ivancheva
Medical University of Varna
Bulgaria

Department of Ophthalmology and Visual Science

Valeri Sheherov
Medical University of Varna
Bulgaria

Department of Ophthalmology and Visual Science

Christina Grupcheva
Medical University of Varna
Bulgaria

Department of Ophthalmology and Visual Science

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