Scientific Online Resource System

Scripta Scientifica Pharmaceutica

Electrochemical detection of Nitrofural in the presence of sodium amminepentacyanoferrate (II)

Iliyan Nikolov Kolev, Sonya J Ivanova, Mihail K Marinov


The present study deals with the development of a new qualitative electrochemical analytic test for detecting the chemotherapeutic agent Nitrofural. The combination of sodium amminepentacyanoferrate(II) and electric power, provided by a 9V battery, was successfully implemented for the analysis of the medicine in question.


Pharmaceutical analysis; Nitrofural; Nitro group; Electrochemical reduction

Full Text


Silverman R; Holladay M. The organic chemistry of drug design and drug action. Amsterdam: Elsevier; 2014.

European Pharmacopoeia, Strasbourg: Council of Europe. 8th ed., vol. 1 and 2; 2013.

Feigl F. Spot Tests In Organic Analysis. Amsterdam: Elsevier; 1966.

Pedersen O. Pharmaceutical Chemical Analysis: Methods for Identification and Limit Tests. Boca Raton: Taylor & Francis Group; 2006.

Kolev IN, Ivanova SJ. The unusual behavior of Gibbs’ reagent versus Nitrofural. Scripta Scientifica Pharmaceutica 2018;5(1): 7-12. DOI:

Bose PK. A New Method for the Detection of the Nitro-Group in some Organic Compounds. Analyst. 1931;56(665):504-7. DOI: 10.1039/AN9315600504.

Fuchigami T, Atobe M, Inagi S. Fundamentals and Applications of Organic Electrochemistry: Synthesis, Materials, Devices. Chichester: John Wiley & Sons; 2014.

Hammerich O; Speiser B. (eds.) Organic Electrochemistry. 5th ed. Boca Raton: Taylor & Francis Group; 2016.

Feigl F, Anger V, Frehden O. Über die Verwendung von Tüpfelreaktionen zum Nachweis von organischen Verbindungen. (IV.) Mikrochemie 1934;15(1):181-9. DOI: 10.1007/bf02789367.

Ohkuma S. A Microdetection of Aromatic Nitro Compounds with Sodium Pentacyanoammineferroate. YAKUGAKU ZASSHI 1955;75(11):1342-45. DOI: 10.1248/yakushi1947.75.11_1342.

Barek J, Cvačka J, Muck A, Quaiserová V, Zima J. Polarographic and Voltammetric Determination of Carcinogenic Nitro and Amino Derivatives of Polycyclic Aromatic Hydrocarbons. Electroanalysis 2001;13(8-9):799-803.

Mishra AK, Gode KD. Electrochemical reduction of nitrofurazone and its determination in pharmaceutical dosage forms by d.c. polarography. Analyst. 1985;110(11):1373-6. DOI: 10.1039/an9851001373.

Reday CS, Reddy SJ. Electrochemical reduction of nitrofurazone and its determination in pharmaceutical dosage forms by differential pulse polarography. Electroanalysis 1992;4(5):595-9. DOI: 10.1002/elan.1140040515.

Hirokazu T, Kazuo M, Keiko T, Saburo N. Fluorometric Analysis with o-Aminothiophenol. V. Fluorometric Determination of Nitrofurazone. YAKUGAKU ZASSHI 1974;94(6):717-23. DOI: 10.1248/yakushi1947.94.6_717.

Guo X, Wang Y, Wu F, Ni Y, Kokot S. The use of tungsten disulfide dots as highly selective, fluorescent probes for analysis of nitrofurazone. Talanta 2015;144:1036-43. DOI: 10.1016/j.talanta.2015.07.055.

Beckman HF. Feed Additives Analysis, Microdetermination of the Medicaments Furazolidone and Nitrofurazone. Journal of Agricultural and Food Chemistry 1958;6(2):130-2. DOI: 10.1021/jf60084a009.

Verma KK, Dubey SK. Detection of aromatic nitro compounds by means of their π-complexes with N,N-diethylaniline. Talanta 1981;28(7):485-6. DOI: 10.1016/0039-9140(81)80074-4.

Feigl F. Spot test detection of nitrocellulose. Chemist-Analyst 1963;52:47.

Jungreis Е, Lipetz V. Sensitive and Selective Spot Test for m-Amino- and m-Nitro-Phenol. Microchimica Acta 1963;51(5-6):886-8. DOI: 10.1007/bf01217613.

Basu K, Chakraborty S, Saha C. A closer look at the Mulliken-Barker test. Resonance 2013;18(9):854-8. DOI: 10.1007/s12045-013-0108-1.

Hearon WM, Gustavson RG. A Semimicro Qualitative Test for the Nitro Group in Organic Compounds. Industrial & Engineering Chemistry Analytical Edition 1937;9(7):352-3. DOI: 10.1021/ac50111a024.



About The Authors

Iliyan Nikolov Kolev
Department of Pharmaceutical Sciences and Pharmaceutical Management, Faculty of Pharmacy, Medical University “Prof. Dr. Paraskev Stoyanov” – Varna, 84 “Tzar Osvoboditel” Blvd., 9000 Varna

Head of education and scientific sector "Pharmaceutical chemistry" Assist. prof. at Department of Pharmaceutical Sciences and Pharmaceutical Management

Sonya J Ivanova
Pharmacy „Poly“, POLIMEKS LTD, Varna


Mihail K Marinov
Freelance electronics engineer, Varna


Font Size