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Management of Reproductive Health Control. PAI-1, MTHFR Mutation and Anovulation

Boryana Atanasova, Aleksandra Dzhepkova, Marina Atanasova, Peter Ivanov, Regina Komsa-Penkova


Introductions: Pregnancy is one unique process, which involves genetic material from the fetus, the mother, and the father. All have their own part with their own physiological particularity. The polycystic ovary syndrome (PCOS) is a heterogeneous endocrinological disorder, which is considered to be the most common cause of infertility. Anovulation in women with PSOC is still not completely understood, that is why the aim of this study is to investigate if there is a real connection between the plasminogen activator inhibitor-1, MTHFR and PSOC or this is only a coincidence.

Materials and Methods: For this study, we engaged 83 women divided into two groups, matched for age and weight, known as case and control. Fifty-eight women with PCOS and 25 weight- and age-matched healthy control volunteers participated in this survey. All participants gave a written informed consent. DNA was isolated from venous blood and PAI-1 4G/5G polymorphism and MTHFR was investigated.

Results: The case group with MTHFR indicator N/M genotype are 48%, followed by N/N - 43%, and M/M - 9%. Controls with N/N are 65%, followed by N/M - 25%, and M/M - 10%. Patients with PAI-1 4G/5G are 47%, followed by 4G/4G - 34% one of the highest numbers, and those with a genotype 5G/5G are 19%. The carrier status in the controls was 17%, 66% and 17% respectively for 5G/5G, 4G/5G, 4G/4G genotype, (or 2.4, ci 0.67 – 9.48, p – ns for carrier status of 4G/4G genotype).

Conclusion: If we could manage our health we should start it as soon as possible. PAI-1 and MTHFR are in a very close connection. Each of them plays a significant role in the human body and health. We can perform a DNA test, watch the levels of homocysteine and B12, manage sport and food. However, our research may serve as a pilot study from which we can conclude that this is only the beginning.


PAI-1, methylene-tetra-hydro-folate-reductase, polycystic ovary syndrome, reproduction


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