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Еxpression of transcriptional factors PAX2 and PAX6 in primate cerebellum related to the age and following ischemiа

Veselina Mihaleva, Dessislava Marinova, Stoyan Pavlov, Meglena Angelova, Vanya Goranova, Tetsumori Yamashima, Anton Tonchev

Abstract

The genes of Pax family play important roles in the proliferation of multiple cell lines, formation of various organs, including the development and organization of the central nervous system. Lately, it was found the significance of two transcriptional factors (ТFs) of this group - pax2 and pax6 in tissue regeneration, especially for Pax6 gene - a novel function in the segmental organization of the hindbrain.

The goal of the present investigation was to study the presence, distribution and dynamics of these two TF in the cerebella of newborn, one-year old and young adult Japanese monkeys (Macaca fuscata), by the latter also after global brain ischemia. According to the age (newborn, one-year old and adult) and period of survival time after ischemia by the adults (9 or 15 days respectively), animals ware distributed in several control and experimental groups. We applied fluorescent immunohistochemical technique for staining cryo-sections of cerebellum for demonstration of pax2 and pax6.

By the newborn animals Pax2(+) cells were found mainly in the inner granular cell layer of the cerebellum and in the layer of Purkinje cells. At later age their quantity was reduced. In the ischemic groups single Pax2(+) cells were located in the Purkinje cell layer and granular cell layer without difference in the location and amount compared with controls.

Pax6(+) cells were found in large amount in all layers of the cerebellar cortex by newborn animals, whereas by one-year old monkeys such cells were found mainly in the inner granular layer. By adult animals of the control group their typical location was only in the granular layer, there was no difference in comparison with the ischemic groups.

The data received conferm and expand the available information related to the presence and typical distribution of these two ТFs in the cerebellum in the course of postnatal development without establishment of changes following global brain ischemia.


Keywords

Pax2, Pax6, малък мозък, примати, исхемия / Key words: Pax2, Pax6, cerebellum, primates, ischemia

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