Scientific Online Resource System

Varna Medical Forum

T-SPOT - TB - A CONTEMPORARY METHOD IN THE IMMUNODIAGNOSIS OF TUBERCULOSIS INFECTION

E. Mehmedova, G. Tsankova, G. Grancharova

Abstract


Tuberculosis (TB) is the first infectious disease declared by the World Health Organization as a global health emergency. It is a chronic disease caused by the Bacillus mycobacterium tuberculosis. TB usually affects the lungs but it can also affect other part of the body such as brain, intestines, kidneys, or the spine. Thus, there are difficulties when diagnosing the disease, in particular, among infants. We aimed at determining the application of T-SPOT for TB in children in the region of Varna for the period of December, 2011 to September, 2012. Data was collected from the Specialized Hospital of Pneumologic and Phthisiatric Diseases of Varna Ltd. T- SPOT - TB test is one of the greatest achievements concerning the diagnosis of TB infection in the recent 100 years. T-SPOT is carried out using two anti- gens - ESAT-6 and CFP-10 as well as cellular cultures from isolated lymphocytes. The production of IFN-ã is measured in ELISPOT. The most important advantage of T-SPOT - TB is its high specificity and sensibility. The test is being successfully applied among children of all ages, particularly in differentiating TB infection from BCG vaccination. During this period in the region of Varna, 12 children were examined, among them two presented with LTBI. Despite the small number of subjects examined, we draw the conclusion that T-spot - TB test plays an important role in the diagnosis of TB, especially in small children.




Keywords

T-SPOT - TB test, tuberculosis in children, diagnosis, differential diagnosis


Refbacks

Font Size


|