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P53 Expression In Colorectal Epithelial Neoplasms And Its Relation To Clinical And Histopathological Features

Nedyalka Zgurova, Maria Tzaneva

Abstract

Introduction: Colorectal carcinoma (CRC) is the result of genetic and epigenetic disorders. The p53 gene, known as the guardian of the genome, plays an important role in apoptosis and inhibition of angiogenesis.

Aim: The aim of our study is to investigate and compare the expression of p53 in the tumor tissue of synchronous/metachronous adenomas and CRC and its relation to clinical and histopathological features, and to evaluate p53 as a predictive marker.

Materials and Methods: Materials from 18 synchronous/metachronous colorectal adenomas and 21 CRCs were fixed in formalin (10% neutral), followed by paraffin embedding of tissue. Tissue sections of 5 μ were stained with hematoxylin and eosin to evaluate histological parameters. For immunohistochemistry, the monoclonal rabbit p53 antibody (Clone Y5) was used. A five-step scale was used to evaluate the percentage of positive nuclear expression: 0 (0-5%), 1 (6-25%), 2 (26-50%) and 3 (51-75%) and 4 (>75%).

Results: Seven (38.89%) of the benign neoplasms were positive for p53 and the expression of the protein was in correlation with size, localization in the right half of the colon and the villous component of the adenoma, and inversely dependent on its differentiation degree (p <0.05). Tumor tissue of 13 (61.90%) CRCs accumulated p53, but there was no correlation with clinical and morphological parameters: age, gender, size, localization, TNM stage and grade of differentiation (p>0.05). The non-neoplastic colonic mucosa was negative in all cases.

Conclusion: Accumulation of p53 is a predictor of malignancy in colorectal adenoma and plays part in adenoma-carcinoma tumor progression.


Keywords

p53, synchronous/metachronous adeno-mas, colorectal carcinoma, clinical-morphological parameters

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