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Izvestia Journal of the Union of Scientists - Varna. Medicine and Ecology Series

The expression of miR-191 - a modern biomarker for distinguishing ulcerative colitis from Crohn's disease

Antonia Atanassova, Maria Teneva

Abstract

Introduction: Literature data shows that miR-191 can be used as a potential diagnostic biomarker to differentiate between Crohn's disease (CD) and ulcerative colitis (UC).

Aim: The aim of the present study is to evaluate the serum expression of miR-191 in patients with chronic inflammatory bowel disease and to determine the role of microRNAs in distinguishing CD from UC.

Materials and Methods: Serum miR-191 expression was tested in 70 patients with inflammatory bowel disease (IBD) (35 with UC, 35 patients with CD) and 30 healthy control subjects through reverse transcriptase and real-time quantitative PCR (RT-qPCR).

Results: The results show that miR-191 has increased expression in CD, active disease, small intestinal localization, and chronic persistent form of UC, and extraintestinal manifestations. The correlation analysis shows a positive correlation between the expression of miR-191 and faecal calprotectin and CDAI.

Conclusion: The serum expression of miR-191 was significantly higher in patients with CD compared to patients with UC. It correlated with the localization and activity in CD, and in UC there was only a correlation to the form of the course and the extraintestinal manifestations. It can be used to distinguish between the two diseases.

Keywords

miR-191; biomarker; Crohn's disease; ulcerative colitis; inflammatory bowel disease

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References

Bi K, Zhang X, Chen W, Diao H. MicroRNAs Regulate Intestinal Immunity and Gut Microbiota for Gastrointestinal Health: A Comprehensive Review. Genes (Basel). 2020 Sep 12;11(9):E1075. doi: 10.3390/genes11091075. PMID: 32932716.

Chapman CG, Pekow J. The emerging role of miRNAs in inflammatory bowel disease: a review. Therap Adv Gastroenterol. 2015;8(1):4–22. doi: 10.1177/1756283X14547360

Li M , Zhao J , Cao M , Liu R , Chen G , Li S , et al. Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells. Biol Res. 2020 Mar 24;53(1):12. doi: 10.1186/s40659-020-00279-2. PMID: 32209121; PMCID: PMC7092522

Lin J, Cao Q, Zhang J, Li Y, Shen B, Zhao Z, et al. MicroRNA expression patterns in indeterminate inflammatory bowel disease. Mod Pathol. 2013;26:148–54. doi: 10.1038/modpathol.2012.131,

Paraskevi A, Theodoropoulos G, Papaconstantinou I, Mantzaris G, Nikiteas N, Gazouli M. Circulating MicroRNA in inflammatory bowel disease. J Crohns Colitis. 2012;6(9):900-4. doi: 10.1016/j.crohns.2012.02.006.

ang L, Zhang R, Chen J, Wu Q, Kuang Z. Baicalin Protects against TNF-α-Induced Injury by Down-Regulating miR-191a That Targets the Tight Junction Protein ZO-1 in IEC-6 Cells. Biol Pharm Bull. 2017;40(4):435-443. doi: 10.1248/bpb.b16-00789.

Wu F, Zhang S, Dassopoulos T, Harris ML, Bayless TM, Meltzer SJ, Brant SR, Kwon JH. Identification of microRNAs associated with ileal and colonic Crohn's disease. Inflamm Bowel Dis. 2010;16(10):1729-38. doi: 10.1002/ibd.21267.

Wu F, Guo NJ, Tian H, Marohn M, Gearhart S, Bayless TM, et al. Peripheral blood microRNAs distinguish active ulcerative colitis and Crohn's disease. Inflamm Bowel Dis. 2011;17(1):241-50. doi: 10.1002/ibd.21450.

Xu XM, Zhang HJ. miRNAs as new molecular insights into inflammatory bowel disease: Crucial regulators in autoimmunity and inflammation. World J Gastroenterol. 2016;22(7):2206-18. doi: 10.3748/wjg.v22.i7.2206.

Zeng Z, Mukherjee A, Zhang H. From genetics to epigenetics, roles of epigenetics in inflammatory bowel disease. Front Genet. 2019;10:1017. doi: 10.3389/fgene.2019.01017.




DOI: http://dx.doi.org/10.14748/isuvsme.v25i1.7200

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