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Photodynamic inactivation of human dental biofilm Isolated Streptococcus Mutans With 2 Photosensitizers - an In Vitro Study

Maya Doychinova, Vesselin Kussovski, Tsvetan Tonchev, Slavcho Dimitrov

Abstract

PURPOSE: The aim of this study is to compare the in vitro antimicrobial effect of 2 photosensitizers: Fo­toSan® medium and ZnPcMe on Streptococcus mutans - biofilm isolated clinical stain

MATERIALS AND METHODS: Twelve patients were instructed to restrain from oral hygiene for 24 hours. Plaque was collected with sterile dental floss, placed in a vial containing 3ml of BFS. Ten-fold dilu­tions of the suspension were made. 0.1ml Samples were plated on Mitis Salivarius agar (1). Bacteria were Gram stained as well and underwent morphological identification. A bacterial suspension at a density of 106 cells/ml. was used. An incubation mixture of 1 ml. bacterial suspension (106 Cl./ml.) and the corresponding PS was prepared, achieving a different final concentration of PS. LED light source with a wavelength of 643 nm was used for irradiation. Samples and controls for each tested PS after 24 h incubation at 37 º C were re­ported.

RESULTS: The control samples in dark showed a minor antimicrobial effect. Samples of bacterial suspen­sion + PS + light irradiation expressed a pronounced antibacterial effect on Streptococcus mutans with small differences in the 2 tested PSs.

CONCLUSIONS: The clinical strain Streptococcus mutans was susceptible to photodynamic inactivation by the two used photosensitizers: FotoSan® medium and ZnPcMe. Dark toxicity is to be neglected. ZnPcMe should undergo further trials and given the positive photodynamic effect in vitro, to be licensed for clinical use. FotoSan®medium should undergo further in vivo and in vitro experiments in order to establish an al­ternative protocol for caries prevention.


Keywords

antibacterial photodynamic therapy; dental caries prevention; Streptococcus mutans

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DOI: http://dx.doi.org/10.14748/ssm.v47i1.1067
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About The Authors

Maya Doychinova
Medical university of Varna
Bulgaria

Faculty of dental medicine

Vesselin Kussovski
Bulgarian academy of science, Stephan Angelov institute
Bulgaria

Tsvetan Tonchev
Medical university of Varna
Bulgaria

Faculty of dental medicine

Slavcho Dimitrov
Medical university of Varna
Bulgaria

Faculty of dental medicine

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