PURPOSE: The aim of this study is to compare the in vitro antimicrobial effect of 2 photosensitizers: FotoSan® medium and ZnPcMe on Streptococcus mutans - biofilm isolated clinical stain
MATERIALS AND METHODS: Twelve patients were instructed to restrain from oral hygiene for 24 hours. Plaque was collected with sterile dental floss, placed in a vial containing 3ml of BFS. Ten-fold dilutions of the suspension were made. 0.1ml Samples were plated on Mitis Salivarius agar (1). Bacteria were Gram stained as well and underwent morphological identification. A bacterial suspension at a density of 106 cells/ml. was used. An incubation mixture of 1 ml. bacterial suspension (106 Cl./ml.) and the corresponding PS was prepared, achieving a different final concentration of PS. LED light source with a wavelength of 643 nm was used for irradiation. Samples and controls for each tested PS after 24 h incubation at 37 º C were reported.
RESULTS: The control samples in dark showed a minor antimicrobial effect. Samples of bacterial suspension + PS + light irradiation expressed a pronounced antibacterial effect on Streptococcus mutans with small differences in the 2 tested PSs.
CONCLUSIONS: The clinical strain Streptococcus mutans was susceptible to photodynamic inactivation by the two used photosensitizers: FotoSan® medium and ZnPcMe. Dark toxicity is to be neglected. ZnPcMe should undergo further trials and given the positive photodynamic effect in vitro, to be licensed for clinical use. FotoSan®medium should undergo further in vivo and in vitro experiments in order to establish an alternative protocol for caries prevention.
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