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Adapted Microassay for Phagocytic Ability of Human Blood Monocytes

H. Divanyan, D. Zanev, B. Galunska

Abstract

The aim of this study was to obtain a pure monocyte population and to examine the phagocytic and bactericidic ability of these cells by using our micromethod for in-vitro assay. We worked out a relatively simple method for examination of the phagocytic ability of monocytes including production of pure monocytes, its adhesion on coverslips and cultivation with autologous or AB-serum and test-bacteria in microcamera constructed by us.

Keywords

Monocytes; Phagocytosis; Index of Phagocytosis; Phagocytic Number; Index of Bactericidity

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References

Диванян, X., Д. Цанев, Л. Франке. Ротатор на култури в епруветки и микрокултури върху покривни стъкла. Per. № 195/86 на ТИС при МА-София.

Диванян, X., Д. Цанев , Л. Франке, К. Божкова. In vitro микрометод за изследване на фагоцитарната и бактерицидна активност на гранулоцити от периферна кръв. - Експерим. мед. морфол., 28, 1989, 49.

Цанев, Д., X. Диванян. Глава на перисталтнчна помпа с приспособление за компенсиране на пулсациите. Per. Ха 252/87 на ТИС при МА-София.

Цанев, Д., X. Диванян. Прибор за изливане на градиента и за извличане на фракции. Рег. № 117/87 на ТИС при МА-София.

Менышикон, Б. Лабораторные методы исследования в клинике. Справочник. Москва, Медицина, 1987, 364 с.

Baran, J., E. Pajdak, М. Isendorf, D. Szmyd. Evaluation of the interactions between strains of S. aureus and peripheral blood phagocytic cells.- Med. Dosw. Mikrobiol., 50, 1998, 131-140.

Baran J, E. Pajdak, M. Isendo r f , D. Szmyd. Evaluation of interactions between strains of S. aureus isolated from different clinical specimens with peripheral blood phagocytic cells.- Med. Dosw. Mikrobiol., 51, 1999, 37-46.

Brodersen, M., P. Burns. The separation of human monocytes from blood including biochemical observation.- Proc. Soc. Exp. Biol. Med., 144, 1973, 941.

Clark, W. The experimental foudations of the modern immunology, New York, John Willey and Sons, 1980, 372.

Cline, M., R. Lehrer . Phagocytosis by human monocytes.- Blood, 32, 1968, 423.

Dekar i s, D. Purification of monocytes.- Clin. Exp. Immunol., 16, 1974, 311.

Eckert, R. Immunzellen.-In: Immunologische Arbeitsmethoden. Jena, VEB Gustav Fischer Verlag, 1984, 253-281.

Jam, L., C. Li, W. Krosby. Cytochemical identifications of monocytes and granulocytes.- Amer. J. Clin. Pathol., 55, 1971, 283.

Kusner, D., J. Adams. ATP-induced killing of virulent Mycooacterium tuberculosis within human macrophages requires phospholipase.- J. Immunol., 164,2000,379-88.

Loos , H., B. Blok-Shu t , R. van Doom, et a l . A method for the recognition and separation of human blood monocytes on density gradients.- Blood, 48,1976,731.

Marodi, L, P. Leijh, R. van Furth. A micromethod for the separate evaluation of phagocytosis and intracellular killing of Staphylococcus aureus by human monocytes and granulocytes.- J. Immunol. Methods, 57, 1983, 353-561.

Raybourne, R., G. Roth, P. Deuster, E. Sternberg, A. Singh. Uptake and killing of Listeria monocytogenes by normal human peripheral blood granulocytes and monocytes as measured by flow cytometry and cell sorting.- FEMS Immunol. Med. Microbiol., 31, 2001, 219-225.

Stuart, A., J. Habeshaw, A. Davidson. Phagocytes in vitro.- In: Handbook of Experimental

Immunology. Vol. 2. Cellular Immunology. D. Weir, ed. Oxford, Blackwell Scientific Publications, 1980, 31.1-31.30.

Suter, E. Multiplication of tuberculous bacilli within mononuclear phagocytes in tissue cultures derived from normal animals and animals vaccinated with BCG.- J. Exp. Med., 97, 1953, 235.

Smith, D., F. Rommel. Vitality of phagocyted cells and bacteria.- J. Immunol. Methods, 17, 1977, 241.

R. van Furth, L. Theda, van Zwe t , C. Leijh. In vitro determination of phagocytosis and intracellular killing by polymorphonuclear and mononuclear phagocytes.- In: Handbook of Experimental Immunology. Vol. 2. Cellular Immunology. D. Weir, ed. Oxford, Blackwell Scientific Publications, 1980, 32.1-32.19.




DOI: http://dx.doi.org/10.14748/ssm.v35i0.1938
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About The Authors

H. Divanyan

Bulgaria

Dept. of Medical Physics, Chemistry and Biology, Prof. P. Stoyanov Medical University of Varna, 55 Marin Drinov Street, BG 9002 Varna, Bulgaria

D. Zanev

Bulgaria

B. Galunska

Bulgaria

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