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Conventional Polychemotherapy of Acute Lymphoblastic Leukemia Patients Associated with Oxidative Stress and Antioxidants Depletion

D. Ivanova, D. Gerova, V. Gerov, L. Gercheva, T. Yankova


The aim of this study was to investigate whether conventional polychemotherapy of acute lymphoblastic leukemia patients contributed to the development of oxidative stress and antioxidants depletion. Plasma levels of thiobarbituric acid-reactive substances assessed by malonedialdehyde (MDA) content were measured as products of lipid peroxidation. Pretreatment MDA values and MDA values during therapy course were estimated to be above the normal range, indicating the occurrence of oxidative stress. Serum iron levels were monitored as a potential source of non-transferrin bound iron with a role in initiation of oxidative burst. Increased serum iron levels were measured during the whole course of chemotherapy. To analyze the effects of cytostatic therapy on the pro-oxidant/antioxidant parameters in plasma we measured the total antioxidant status (TAS) and a single plasma antioxidant - uric acid (UA). A significant reduction of TAS levels was found at the end of the therapy course, strongly correlating with UA content (r=0,9; p<0,05). Our data suggest that uric acid as a routine laboratory indicator could also serve as a marker of blood antioxidant capacity.


Acute Lymphoblastic Leukemia; Total Antioxidant Status; Uric Acid; Lipid Peroxidation; Total Serum Iron

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About The Authors

D. Ivanova


D. Gerova


D. Gerova, Dept. of Preclinical, Clinical Pharmacology and Blochemistiy, Prof. P. Stoyanov Medical University of Varna. 55 Marin DrinovStr. BG-9002, BULGARIA

V. Gerov


L. Gercheva


T. Yankova


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