The aim of this study was to investigate whether conventional polychemotherapy of acute lymphoblastic leukemia patients contributed to the development of oxidative stress and antioxidants depletion. Plasma levels of thiobarbituric acid-reactive substances assessed by malonedialdehyde (MDA) content were measured as products of lipid peroxidation. Pretreatment MDA values and MDA values during therapy course were estimated to be above the normal range, indicating the occurrence of oxidative stress. Serum iron levels were monitored as a potential source of non-transferrin bound iron with a role in initiation of oxidative burst. Increased serum iron levels were measured during the whole course of chemotherapy. To analyze the effects of cytostatic therapy on the pro-oxidant/antioxidant parameters in plasma we measured the total antioxidant status (TAS) and a single plasma antioxidant - uric acid (UA). A significant reduction of TAS levels was found at the end of the therapy course, strongly correlating with UA content (r=0,9; p<0,05). Our data suggest that uric acid as a routine laboratory indicator could also serve as a marker of blood antioxidant capacity.
Abdel -Aziz, A. F., M. M. El -Nggar . Superoxide dismutase activities in serum and white blood cells of patients with some malignancies.- Cancer Lett., 113, 1997, 61-64.
Becker , B. F . Towards the physiological function of uric acid.- Free Rad. Biol Med., 14, 1993, 615-631.
Devi , G. S., M. H. Prasad, 1. Saraswathi, D. Raghu, D. N. Rao, P. P. Redd y. Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias.- Clin. Chim. Ada , 293, 2000, 53-62.
Durken, M. , C. Herrnring, B. Finck, S. Nagel , P. Niel sen, R. Fischer, H. M. Berger, R. M. Moison, U. Pichlmeier, B. Kohl schut ter , A. R. Zander , A. Kohlschutter. Impaired plasma antioxidative defense and increased nontransferrin-bound iron during high-dose chemotherapy and radiochemotherapy preceding bone marrow transplantation.- Free Rad. Biol. Med., 28, 2000, 887-894.
Emily, S., J. A. Williams, R. M. Hinson, S. Senturker , Y. Lee. Oxidative stress interferes with cancer chemotherapy: inhibition of lymphoma cell
apoptosis and phagocytosis.- Blood, 96, 2000, 307-313.
Erhola, M. , P. Kellokumpu-Lehtinen, T. Metsa-Ketela, K.. Alanko, M. M. Nieminen. Effects of anthracyclin-based chemotherapy on total
plasma antioxidant capacity in small celllung cancer patients.- Free Rad. Biol Med., 21, 1996, 383-390.
Floyd, R. A. Role of oxygen free radicals in carcinogenesis and brain ischemia.- FASEBJ., 4, 1990, 2587-2597.
Ghalaut , V. S., P. S. Ghalaut, S. Singh. Lipid peroxidation in leukaemia.- J. Assoc. Physicians India, 47, 1999, 403-405.
Gonenc, A. , Y. Ozkan, M. Torun, B. Simsek. Plasma malonedialdahyde (MDA) levels in breast and lung cancer patients.- J. Clin. Pharm.
Ther., 26, 2001, 141-144.
Hickman, J. A. Apoptosis induced by anticancer drugs.- Cancer Metast. Rev., 11, 1992, 121-139.
Ihara. H. , N. Hashizume, T. Hasegawa, M. Yoshida. Antioxidant capacities of ascorbic acid, uric acid, alpha-tocopherol, and bilirubin can be measured in the presence of another antioxidant, serum albumin.- J. Clin. Lab. Anal., 18, 2004, 45-49.
Kumaraguruparan, R., R. Subapriya, P. Viswanathan, S. Nagi i. Tissue lipid peroxidation and antioxidant status in patients with adenocarcinoma of the breast.- Clin. Chim. Acta, 325, 2002, 165-170.
Look, M.P. , E. Musch. Lipid peroxides in the poly chemotherapy of cancer patients.- Chemotherapy, 40, 1994, 8-15.
Miller, N. J., C. Rice-Evans , M. J. Davies, V. Gopinathan, A. Milner . A novel method for measuring antioxidant capacity and its application to
monitoring the antioxidant status in premature neonates.- Clin. Sei., 84, 1993, 407-412.
Monteiro Gil , O. , N. G. Oliveira, A. S. Rodrigues, A. Laires, T. C. Ferreira, E. Limbert , A. Leonard, G. Gerber , J. Rueff. Cytogenetic alterations and oxidative stress in thyroid cancer patients after iodine-131 therapy.- Mutagenesis, 15, 2000, 69-75.
Pham, N. A., D. W. Hedley. Respiratory chain-generated oxidative stress following treatment of leukemic blasts with DNA-damaging agents.- Exp. Cell Res., 264. 2001,345-348.
Porter, N. A. , J. Nixon, R. Isaac. Cyclic peroxides and the thiobarbituric assay.- Biochim. Biophys. Acta, 441, 1976, 506-512.
Email this article (Login required) 
