The role of cold immersion stress for the free-radical mediated lipid peroxidation in rat plasma and erythrocytes was studied. A model of cold-induced stress in rats was created. A significant increase of the level of thiobarbituric acid-reactive substances (TBARS) in plasma was found after chilling. Activated catabolic processes and xanthine-oxidase reactions during hypoxia could cause the increase measured in plasma levels of uric acid one hour after cold stress. A decrease in the antioxidant defence measured by uric acid consumption in rat blood plasma took also place already at the 3rd hour after chilling. These effects were not accompanied neither by elevated levels of TBARS, nor by a decrease in the reduced glutathione or elevated ratio of oxidized/ total glutathione in erythrocytes. The results indicated that lipid peroxidation was a component of the cold-induced stress in rats.
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