The intensive development of molecular biology and biochemistry in recent years has allowed the disclosure of intimate mechanisms of a number of processes in the cell. Numerous molecules have become useful tools for diagnosing and predicting a number of diseases. These are, for example, the small, non-coding micro RNAs (miRNAs). It was found that the majority of protein-coding genes contain miRNA-binding sites under selective pressure (1) that make them the master regulators of cellular processes. Thus
Friedman RC, Farh KK, Burge CB, Bartel DP. Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 2009;19(1):92-105. doi: 10.1101/gr.082701.108
Chang TC, Mendell JT. microRNAs in vertebrate physiology and human disease. Annu Rev Genomics Hum Genet. 2007;8:215-39. doi: 10.1146/annurev.genom.8.080706.092351
Witwer KW, Buzás EI, Bemis LT, Bora A, Lässer C, Lötvall J, et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research. J Extracell Vesicles. 2013;2. doi:10.3402/jev.v2i0.20360.
Chen X, Ba Y, Ma L, Cai X, Yin Y, Wang K, et al. Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases. Cell Res. 2008;18(10):997-1006. doi: 10.1038/cr.2008.282
D`Alessandra Y, Devanna P, Limana F, Straino S, Di Carlo A, Brambilla PG, et al. Circulating microRNAs are new and sensitive biomarkers of myocardial infarction. Eur Heart J. 2010;31(22):2765-73. doi: 10.1093/eurheartj/ ehq16
Witwer KW, Sarbanes SL, Liu J, Clements JE. A plasma microRNA signature of acute lentiviral infection: biomarkers of CNS disease. AIDS. 2011;25(17):2057-67. doi: 10.1097/QAD.0b013e32834b95bf
von Ahlfen S, Missel A, Bendrat K, Schlumpberger M. Determinants of RNA quality from FFPE samples. PLoS One. 2007;2(12):1261. doi: 10.1371/journal.pone.0001261