The process of training of tennis players creates anatomical differences between dominant and non-dominant upper limbs and leads to an asymmetry in the muscle mass distribution. The aim of the study is to estimate the degree of muscle mass asymmetry between the dominant and non-dominant upper limbs in young tennis players, using multi-frequency bioelectrical impedance measurements. The study sample included 14 male tennis players and 11 school children aged 12 years. Segmental analysis of body composition was conducted by a bioelectrical impedance analyzer (model: InBody 170). The athletes had a higher muscle mass of the dominant upper limb compared to the non-dominant. The non-athlete boys were characterized by lower asymmetry coefficient level of the muscle mass of the upper limbs compared to the tennis players (p<0.05). No significant relationship between the asymmetry coefficients of the upper limbs, mean age and years of training was found in tennis players.