According to the original S10 methodology, the processing of biological material takes place at low temperature (-30oC) and vacuum (10-3-10-6 Torr). From the creation of the method until today, the S10 plastination technique has changed significantly.
Injection plastination by Shawnda et al. (2013) is a new economical method for producing of elastic, life-like and safe anatomical preparations. The impregnation is carried out at room temperature, at which Biodur S10 is a liquid, and normal atmospheric pressure.
The Brazil POLISIL® Silicones products allow impregnation to take place at room temperature. The final products are dry, with reliable structures, odourless, durable, with good flexibility and preserve the anatomical structures.
A modified room temperature plastination technique by Ottone et al. (2016) is used in South America. Three-dimensional reconstructions were obtained from plastinated specimens. Further three-dimensional and two-dimensional cuts were achieved.
According to Starchik (2016) the average shrinkage for tissue cores plastinated by the room-temperature technique was 1.5 times less than the cold-temperature method (p < 0.05). The total duration of impregnation and curing stages of core samples for cold-temperature plastination proved to be 1.54 times longer than those of the room-temperature technique.
Conclusion: Impregnation at room temperature and low pressure is a promising method for plastering of anatomical objects. It is cheaper, faster and more economical.