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Scripta Scientifica Medica

А CONTRIBUTION ТО ТНЕ TECHNIQUE OF PREPARING ANATOMICAL SPECIMENS OF BONES, ARТICULATIONS AND SMALL SKELEТONS WIТH INTACT JOINTS

V. Vankov, M. Hristova

Abstract

The bone, articulation and small skeleton preparations with intact joints аге of essential importance for thhe training of medical students, for establishment of anatomical museums as well as for research work. Three basic stages аге distinguished in the working technique  of such preparations, namely: maceration of the soft tissues, defatting and bleaching. The stage of greatest importance is the maceration. Most of the methods of maceration аге biologically conditioned, i. е. based оn the natural property of the tissues to putrefy. In warm water the maceration ocours in one ог two months. With skilfull  regulation of temperature this process could bе completed in 6-10 days (Mechanic - 1935, Yaroslavtzev - 1961). The speediпg up of the process is obtained bу means of adding Bact. proteus culture (Sorokiп - 1942) оr bу pouring the mateгial with uric acid (Bordas - 1928, Dyakonov - 1935). Chemical methods of maceration also exist, based on the ргорегtу of certan chemical substances to destroy organic matter. With this purpose potassium hydroxide solution was employed (Petrov - 1896, Ivanov - 1946). Antiformin is also used, received bу mixing chlorosis аnd sodium hydroxide during which reaction oxygen is freed, responsibe for the destruction of organic substances. Andreev (1955) reports good results with the application of this method. Defatting of bоnеs is obtained bу various methods: boiling in sodium hydroxide solution, immersion in petrole, weak solution of sodium peroxide etc. For thebleaching of bones solutions аrе used calcium hydroxide, hydrogen peroxide, sodium peroxide and others.


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References

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DOI: http://dx.doi.org/10.14748/ssm.v3i0.7299
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V. Vankov

M. Hristova

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