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Scripta Scientifica Medica

ВЪРХУ НЯКОИ СВОЙСТВА НА ФИТОХЕМАГЛУТИНИНА ОТ PHASEOUS VULGARIS И НЕГОВОТО ПРИЛОЖЕНИЕ В МЕДИЦИНСКАТА ЦИТОГЕНЕТИКА

М. Цонева-Манева

Abstract

The results are reported of the study of certain chemical and biological properties of the extracted from the seeds of Phaseolus vulgaris, Sax type, phytohemagglutinin. The preparation isolated after the Rigas and Osgood method, represents а white amorphous substance, insolble in distilled water and readily solved in physiological solution.The spectral characteristics of the preparation reveals its protein nature, and the paper electrophoresis shows that it consists of two fractions: оnе is situated in the y-globulin region, and the other – in the globulin region of blооd serum.

It is established that the preparation obtained displays а strongly pronounced hemagglutination properties towards the erythrocytes of man, rabblt, mouse, rooster and frog. Furthermore, the preparation shows strongly manifested cytohemagglutination properties with regard to certain cells of normal and malignant nature.

The preparation obtained has the property of stimulating the division of monocytes and lymphocytes in the peripheralblооd of man, on account of which it is very appropriate for use in obtaining cultures for cytogenetics analysis.


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References

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DOI: http://dx.doi.org/10.14748/ssm.v2i0.7357
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М. Цонева-Манева

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