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Effects of Aronia melanocarpa fruit juice on lipid and bone metabolism in ovariectomized rats

Antoaneta Georgieva, Milena Todorova, Miroslav Eftimov, Krasimir Kuzmanov, Vasilena Kuzmanova, Atanas Kuzmanov, Mila Vlaskovska, Stefka Valcheva-Kuzmanova


Introduction: The ovariectomized (OVX) rat is a gold-standard model to mimic the changes in the female organism during menopause. Aronia melanocarpa fruits are very rich in polyphenols and exert many beneficial effects in animal models and clinical trials.

Aim: The aim of the present study was to investigate the effects of Aronia melanocarpa fruit juice (AMFJ) on lipid metabolism and bone mineral density (BMD) in OVX rats.

Materials and Methods: Four groups of female Wistar rats were formed, each consisting of 14 animals—sham operated (SO), OVX (treated with distilled water), as well as OVX+AMFJ5, and OVX+AMFJ10 (OVX treated with 5 and 10 mL/kg AMFJ, respectively). The animal treatment began 2 weeks after the operation. After a three-month treatment period, body weight (BW), total fat (TF) and retroperitoneal fat (RPF), as well as serum total cholesterol were measured. The indices TF/BW and RPF/BW were calculated. Femur BMD was determined as well. The statistical analysis used was one-way ANOVA.

Results: Compared to SO animals, OVX rats showed a statistically significant increase in TF (p < 0.05), RPF (p < 0.01), TF/BW (p < 0.05), and RPF/BW (p < 0.01). Plasma cholesterol levels of OVX animals were increased and their BMD was decreased if compared to SO rats, but these changes were not statistically significant. Compared to OVX rats, AMFJ did not affect lipid accumulation and cholesterol levels but at the dose of 10 mL/kg significantly increased BMD (p < 0.05 vs. OVX).

Conclusion: Aronia melanocarpa fruit juice was not able to prevent the fat accumulation in OVX rats, but managed to restore their BMD.


Aronia melanocarpa, polyphenols, lipid metabolism, bone mineral density, ovariectomy, rats

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About The Authors

Antoaneta Georgieva
Medical University of Varna

Department of Pharmacology and Clinical Pharmacology and Therapeutics, Faculty of Medicine

Milena Todorova
Medical University of Varna

Department of Pharmacology and Clinical Pharmacology and Therapeutics, Faculty of Medicine

Miroslav Eftimov
Medical University of Varna

Department of Pharmacology and Clinical Pharmacology and Therapeutics, Faculty of Medicine

Krasimir Kuzmanov
Medical University of Varna


Vasilena Kuzmanova
Medical University of Varna

Student, Faculty of Dental Medicine

Atanas Kuzmanov
Medical University of Varna

Student, Faculty of Dental Medicine

Mila Vlaskovska
Medical University of Sofia

Department of Pharmacology and Toxicology, Faculty of Medicine

Stefka Valcheva-Kuzmanova
Medical University of Varna

Department of Pharmacology and Clinical Pharmacology and Therapeutics, Faculty of Medicine

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