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Scripta Scientifica Medica

Tissue clearing protocols: an overview of current methods and approaches

Andreas Kontny, Pavel Pavlov, Alexander Zlatarov, Turgay Kalinov, Anton B Tonchev

Abstract

Observation of complex cellular shapes in three-dimensional (3D) tissue context requires the use of thick blocks of tissue samples, which are not transparent. To overcome this optical opacity, a specific set of histological techniques have been developed and named tissue clearing methods. Here we review the major available tissue clearing protocols and justify the advantages of a particular protocol of tissue clearing named CUBIC over the other methods in imaging and 3D reconstruction.


Keywords

tissue clearing, 3D microscopy, CUBIC protocol

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References

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DOI: http://dx.doi.org/10.14748/ssm.v54i2.8141
About The Authors

Andreas Kontny
Medical University of Varna
Bulgaria

Department of Anatomy and Cell Biology, Faculty of Medicine

Pavel Pavlov
Medical University of Varna
Bulgaria

Department of Anatomy and Cell Biology, Faculty of Medicine

Alexander Zlatarov
Medical University of Varna
Bulgaria

Department of General and Operative Surgery, Faculty of Medicine

Turgay Kalinov
Medical University of Varna
Bulgaria

Department of General and Operative Surgery, Faculty of Medicine

Anton B Tonchev
Medical University of Varna
Bulgaria

Department of Anatomy and Cell Biology, Faculty of Medicine

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