Comparative electron microscopic and immunohistochemical study of stromal cells in giant cell tumor of bone

Georgi P. Georgiev; Boycho Landzhov; Svetoslav A. Slavchev; Pavel Rashev; Todor Todorov; Lina Malinova; Luben Stokov; Wladimir Ovtscharoff

doi:10.14748/ssm.v45i0.832

Comparative electron microscopic and immunohistochemical study of stromal cells in giant cell tumor of bone

Georgi P. Georgiev, Boycho Landzhov, Svetoslav A. Slavchev, Pavel Rashev, Todor Todorov, Lina Malinova, Luben Stokov, Wladimir Ovtscharoff

Abstract

Giant cell tumor of bone is an osseous neoplasm that is histologically benign but clinically shows local aggression and high rate of recurrence. The histogenesis of this lesion remains unclear. The histological appearance does not predict the clinical outcome and there are still many unanswered questions with regard to both its treatment and prognosis. In order to further clarify this lesion, we examined ultrastructurally and immunohistochemically the tumor mononuclear cells in ten patients operated on in our hospital for matrix metalloproteinase-9. Positive reaction was detected in the spindle-like stromal cells of giant cell tumor of bone and these cells had the ultrastructural characteristics of fibroblastic cells. The other mononuclear cells did not express matrix metalloproteinase-9 and showed ultrastuctural characteristics of macrophage-like cells. The positive reaction for matrix metalloproteinase-9 in all patients clearly shows that this protease may play a key role in the pathophysiology of giant cell tumor of bone.

Keywords

giant cell tumor of bone; ultrastructural study; matrix metalloproteinase-9

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DOI: http://dx.doi.org/10.14748/ssm.v45i0.832

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