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Expression of transcription factor Coup-TF1 (NR2F1) in developing occipital cortex in humans

Meglena Angelova, Radoslav Minkov, Vanya Goranova, Stoyan Pavlov, Vesselina Michaleva, Anton B. Tonchev

Abstract

PURPOSES: We aimed to investigate the presence, distribution and abundance of transcription factor (TF) coup-tf1 (also known as NR2F1) in the germinative zones of human telencephalon during the fetal period. This transcription factor is of significance for the normal neuronal migration and differentiation of projection neurons in the mouse forebrain.

MATERIALS AND METHODS: Brain tissue samples from spontaneously aborted human fetuses aged between 12  and 28  gestational weeks (g.w.) were fixed in paraformaldehyde and histologically investigated. Sections immunostained for coup-tf1 were scanned and its expression in different zones of the occipital lobe of developing pallium was evaluated. COUP-TF1 positive cells were counted and their percentage of all DAPI-stained nuclei was calculated in order to establish the abundance of the COUP-TF1-expressing cells among the total cell population.

RESULTS: COUP-TF1 expression was prominent in all investigated zones of the developing human occipital lobe at stage 17 g.w. In all zones studied, COUP-TF1 positive cells presented a relatively high fraction (~71-95 %) of the cells counted with the highest value of 95.24% in the ventricular zone.

CONCLUSIONS: The present data on the location, abundance and distribution pattern of coup-tf1-expressing cells in the human occipital lobe provide information on the possibility that this TF might participate to human to corticogenesis, similarly to the mouse. 


Keywords

COUP -TF1; NR2F1; cortical progenitors; human fetal telencephalon

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DOI: http://dx.doi.org/10.14748/ssm.v47i1.851
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About The Authors

Meglena Angelova
Medical University of Varna
Bulgaria

Department of Anatomy, Histology and Embryology

Radoslav Minkov
Medical University of Varna
Bulgaria

University Hospital `Prof. Stamatov` - Varna, Bulgaria

Vanya Goranova
Medical University of Varna
Bulgaria

Department of Anatomy, Histology and Embryology

Stoyan Pavlov
Medical University of Varna
Bulgaria

Department of Anatomy, Histology and Embryology

Vesselina Michaleva
Medical University of Varna
Bulgaria

Anton B. Tonchev
Medical University of Varna
Bulgaria

Department of Anatomy, Histology and Embryology

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