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Effect of aqueous infusion from Cotinus coggygria leaves on indomethacin-induced gastric mucosal damage and oxidative stress in rats

Danail Pavlov, Diana Ivanova, Miroslav Eftimov, Maria Tzaneva, Milka Nashar, Ina Kobakova, Stefka Valcheva-Kuzmanova


Purpose: The Smoke tree (Cotinus coggygria) is well known medicinal plant that is used mainly externally by the Balkan folk medicine for its antiseptic and anti-inflammatory properties. There are scarce reports about the internal usage of decoctions from C. coggygria leaves against gastric ulcers. Our study was aimed to explore the effect of aqueous infusion from Cotinus coggygria leaves (AICCL) on indomethacin-induced gastric mucosal damage in Wistar rats and its possible effect on the gastric oxidative status.

Material and Methods: Three AICCL (1/100, 2/100 and 4/100) were applied by gastric gavage (volume: 10 ml/kg) as a pretreatment 3 days before a single intragastric administration of indomethacin (dose: 100 mg/kg). Gastric ulcer formation was estimated morphometrically and histopathologically 4 h after the indomethacin administration. Malondialdehyde (MDA) in blood serum and stomach was measured as a biochemical marker of lipid oxidation. Gastric necrosis was also evaluated by alkaline phosphatase (ALP) and uric acid (UA) assays.

Results: Morphometrical examinations of stomachs showed that the 2/100 AICCL significantly decreased the ulcer number and area. Histopathological studies demonstrated that AICCL induced a reduction of the depth and severity of indomethacin-induced mucosal lesions. AICCL reduced the elevated by indomethacin gastric MDA, ALP and UA levels.

Conclusion: Indomethacin-induced gastric mucosal damage was accompanied by the development of oxidative stress. AICCL-pretreatment alleviated the gastric lesions, and reduced the indomethacin-induced elevation of ALP and UA. It could be suggested that the gastroprotective effect of AICCL was due to its antioxidant properties as evidenced by the decreased gastric MDA levels.


Cotinus coggygria; Indomethacin; Gastric mucosal damage; Oxidative stress; Lipid peroxidation; Antioxidant activity

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About The Authors

Danail Pavlov
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Diana Ivanova
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Miroslav Eftimov
Medical University of Varna

Department of Preclinical and Clinical Pharmacology

Maria Tzaneva
Medical University of Varna

Department of Preclinical and Clinical Sciences

Milka Nashar
Medical University of Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Ina Kobakova
Medical University of Varna

Department of General and Clinical Pathology, Forensic Medicine and Deontology

Stefka Valcheva-Kuzmanova
Medical University of Varna

Department of Preclinical and Clinical Pharmacology

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