Fat-soluble vitamins content (all-trans-retinol, alpha-tocopherol and cholecalciferol) in edible tissue of Bluefish (Pomatomus saltatrix), a typical Black sea pelagic fish, and in Rainbow trout (Oncorhynchus mykiss), a typical farmed freshwater fish, were determined and compared on raw state and after frozen storage. The sample preparation procedure includes saponification and consequent extraction of fat-soluble vitamins with n-hexane.
The extract was dried under nitrogen flow and redissolved in methanol. HPLC analysis of methanolic samples was performed on ODS2 Hypersil (250x4,6, 5um) column with a mobile phase of methanol:water = 97:3. The quantification of fat-soluble vitamins was by the method of standard addition. Retinol and cholecalciferol were monitored by UV detection and alpha-tocopherol was detected by fluorescence.
The retinol and cholecalciferol contents in fresh edible tissue of Black sea Bluefish (38.5±2.4 μg.100g-1ww and 11.2±1.2 μg.100g-1ww, respectively) were close to values in the freshwater fish Rainbow trout (58.9±2.6 μg.100g-1ww and 14.9±1.1 μg.100g-1ww, respectively). Alpha-tocopherol content was several fold higher in Rainbow trout (1648.9±68.8 μg.100g-1ww) than in Black sea Bluefish (427.1±37.1 μg.100g-1ww).
Long period of storage affected mostly retinol and alpha-tocopherol contents in two fish species. While cholecalciferol content remained almost unchanged.
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