Purpose: The aim of this study is to investigate and compare the in vitro antimicrobial effect of 5 photosensitizers: methylene blue(MB), FotoSan® and 3 types of methalphtalocyanines on the reference strain Streptococcus mutans.
Materials and methods: A bacterial suspension at a density of 106 cells / ml. is used. An incubation mixture of 1 ml. bacterial suspension (106 Cl./ml.) and the corresponding PS is prepared, achieving a final concentration of PS 1-6 μl. Controls: bacterial suspension in the dark; with irradiation, bacterial suspension + PS in the dark. Sample: bacterial suspension + PS + irradiation. Diode laser with a wavelength of 600 nm is used for irradiation. There are also cultures from controls and samples after preliminary 10-fold dilutions (106; 105; 104; 103; 102;) 6 samples and controls for each tested PS after 24 h incubation at 37º C are reported.
Results: The control samples in dark and toxicity only after laser radiation laser show a weak antimicrobial effect. Exception for the Ga(ІІІ)-phtalocyanines-serious dark toxicity. Samples of bacterial suspension + PS + diode laser irradiation showed strong antibacterial effect on St. Mutans with small differences in all tested PSs.
Conclusion: The conducted testing demonstrated that the six tested PS show strong antimicrobial effect in the strain reference of S. Mutans, with some small differences between the tested PS.
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