Scientific Online Resource System

Scripta Scientifica Medica

Antidepressant-like effect of aronia melanocarpa fruit juice applied subchronically to rats

Miroslav Eftimov, Stefka Valcheva-Kuzmanova


PURPOSE: Polyphenols are bioactive substances found in many plants. Aronia melanocarpa fruits are one of the richest sources of polyphenols, amongst them proanthocyanidins, flavonoids and phenolic acids. Some of the polyphenols are reported to cross the blood-brain barrier and thus they can act centrally. The aim of the present study was to investigate the effect of Aronia melanocarpa fruit juice (AMFJ) on depressive- like behavior in subchronically treated (21 and 30 days) male Wistar rats utilizing the forced swim test (FST).

MATERIAL AND METHODS: AMFJ was applied orally through an orogastric cannula once daily at doses of 2,5 ml/kg, 5 ml/kg and 10 ml/kg for periods of 21 and 30 days to the respective experimental groups. The FST was carried out 1 hour after the treatment on the 21st and 30th day.

RESULTS: AMFJ applied for 21 days decreased significantly (p < 0,05) the immobility time in the FST at the doses of 2,5 ml/kg and 10 ml/kg. In rats treated with AMFJ for 30 days, the immobility time was dose-dependently decreased and at the dose of 10 ml/kg it was significantly lower (p < 0,05) than the control one.

CONCLUSION: The decreased immobility time in the FST suggests an antidepressant-like effect of AMFJ in rats which could be due to the polyphenolic ingredients of the juice.


Aronia melanocarpa; forced swim test; behavior; antidepressant; male rats

Full Text


Andres-Lacueva, C., B. Shukitt-Hale, R. L. Galli, O. Jauregui, R. M. Lamuela-Raventos, J. A. Joseph. Anthocyanins in aged blueberry-fed rats are found centrally and may enhance memory.- Nutr. Neurosci., 8, 2005, No 2, 111-120.

Anjaneyulu, M., K. Chopra, I. Kaur. Antidepressant activity of quercetin, a bioflavonoid, in streptozotocin- induced diabetic mice.- J. Med. Food, 6, 2003, No 4, 391-395.

Butterweck V. Mechanism of action of St John`s wort in depression : what is known?- CNS Drugs., 17, 2003, No 8, 539-62.

Dutta, A. K., B. Ghosh, S. Biswas, M. E. A. Reith. D-161, a novel pyran-based triple monoamine transporters blocker: Behavioral pharmacological evidence for antidepressant-like action.- Eur J Pharmacol., 589, 2008, No 1-3, 73-79.

Howell, A. B., J. D. Reed, C. G. Krueger, R. Winterbottom, D. G. Cunningham, M. Leahy. A-type cranberry proanthocyanidins and uropathogenic bacterial anti-adhesion activity.- Phytochemistry, 66, 2005, No 18, 2281-2291.

Janle, E. M., M. A. Lila, M. Grannan, L. Wood, A. Higgins, G. G. Yousef, R. B. Rogers, H. Kim, G. S. Jackson, L. Ho, C. M. Weaver. Pharmacokinetics and tissue distribution of 14C-labeled grape polyphenols in the periphery and the central nervous system following oral administration.- J. Med. Fd, 13, 2010, No 4, 926-933.

Linde, K., L. Knüppel. Large-scale observational studies of hypericum extracts in patients with depressive disorders-a systematic review.- Phytomedicine, 12, 2005, No 1-2, 148-157.

Machado, D. G., L. E. B. Bettio, M. P. Cunha, A. R. Santos, M. G. Pizzolatti, I. M. Brighente, et al. Antidepressant-like effect of rutin isolated from the ethanolic extract from Schinus molle L. in mice: Evidence for the involvement of the serotonergic and noradrenergic systems.- Eur. J. Pharmacol., 587, 2008, No 1-3, 163-168.

Müller, W. E. Current St John`s wort research from mode of action to clinical efficacy.- Pharmacol. Res., 47, 2003, No 2, 101-109.

Nöldner, M., K. Schötz. Rutin is essential for the antidepressant activity of Hypericum perforatum extracts in the forced swimming test.- Planta Med., 68, 2002, No 7, 577-580.

Olsen, H. T., G. I. Stafford, J. van Staden, S. B. Christensen, A. K. Jäger. Isolation of the MAO-inhibitor naringenin from Mentha aquatica L.- J. Ethnopharmacol., 117, 2008, No 3, 500-502.

Oszmianski, J., A. Wojdylo. Aronia melanocarpa phenolics and their antioxidant activity.- Eur. Food Res. Technol., 221, 2005, No 6, 809-813.

Porsolt, R. D. Animal model of depression.- Biomedicine, 30, 1979, No 3, 139-140.

Rangel-Ordonez, L., M. Noldner, M. Schubert-Zsilavecz, M. Wurglics. Plasma levels and distribution of flavonoids in rat brain after single and repeated doses of standardized Ginkgo biloba Extract EGb 761.- Planta Med., 76, 2010, No 15, 1683-1690.

Rocha, F. F., M. T. Lima-Landman, C. Souccar, M. M. Tanae, T. C. De Lima, A. J. Lapa. Antidepressant- like effect of Cecropia glazioui Sneth and its constituents - in vivo and in vitro characterization of the underlying mechanism.- Phytomedicine, 14, 2007, No 6, 396-402.

Singleton, V., J. Rossi. Colorimetry of total phenolic with phosphomolibdiphosphotungstic acid reagents.- Am. J. Enol. Viticult., 16, 1965, 144-158.

Takeda, H., M. Tsuji, M. Inazu, T. Egashira, T. Matsumiya. Rosmarinic acid and caffeic acid produce antidepressive-like effect in the forced swimming test in mice.- Eur. J. Pharmacol., 449, 2002, No 3, 261-267.

Valcheva-Kuzmanova, S., M. Eftimov, P. Denev, M. Krachanova, A. Belcheva. Effect of Aronia melanocarpa fruit juice on alcohol-induced depressivelike behavior in rats.- Scri. Sci. Med., 45, 2013, Suppl. 4, 25-31.

Willis, L. M., B. Shukitt-Hale, J. A. Joseph. Recent advances in berry supplementation and age-related cognitive decline (Note).- Curr. Opin. Clin. Nutr. Metab. Care, 12, 2009, No 1, 91-94.

Xia, X., G. Cheng, Y. Pan, Z. H. Xia, L.D. Kong. Behavioral, neurochemical and neuroendocrine effects of the ethanolic extract from Curcuma longa L. in the mouse forced swimming test.- J. Ethnopharmacol., 110, 2007, No 2, 356-363.

Xu, C., L. Luo, R. X. Tan. Antidepressant effect of three traditional Chinese medicines in the learned helplessness model.- J. Ethnopharmacol., 91, 2004, No 2-3, 345-349.

Article Tools
Email this article (Login required)
About The Authors

Miroslav Eftimov
Medical University of Varna

Department of Preclinical and Clinical Pharmacology

Stefka Valcheva-Kuzmanova


Font Size