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Cytoprotective and cytoproliferative effects of ethanolinfusions from Morus nigra and Cotinus coggygria heartwood in 3T3-L1 preadipocytes

Milena Pasheva, Danail Pavlov, Oskan Tasinov, Milka Nashar, Yoana Kiselova-Kaneva, Diana Ivanova

Abstract

PURPOSE: The purpose of the present study was to examine the effect of two ethanol infusions from the heartwood of mulberry tree (Morus nigra) and smoke tree (Cotinus coggygria) on viability of preadipocyte cells. MATERIAL AND

METHODS: Preadypocyte 3T3-L1 cells were treated with 40% ethanol infusions from the heartwood of both species, as well as with 40% ethanol as a control. MTT-test was applied to assess cell viability.

RESULTS: Ethanol infusions increased cell viability as compared to ethanol-treated controls. Along with the cytoprotective effect, also cytoproliferative effect was established for both infusions.

CONCLUSION: Estimated cytoprotective percentage values of infusion in the nutrient medium could be used in further investigations aimed to explore biological effects of ethanol heartwood infusions from M. nigra and C. coggygria.


Keywords

Morus nigra; Cotinus coggygria; heartwood infusions; MTT-test; 3T3-L1 preadipocytes; cell viability

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References

Chan, K .C., M.Y.Yang, M. C. Lin, Y. J. Lee, W.C. Chang, C. J. Wang. Mulberry leaf extract inhibits the development of atherosclerosis in cholesterolfed rabbits and in cultured aortic vascular smooth muscle cells.- J. Agric Food Chem.,61, 2013, 11, 2780.2788.

Dulger, B., N. Hacioglu, S. Bilen. Antimicrobial activity of Cotinus coggyria from Turkey.- Asian J. Chem., 21, 2009, No. 5, 4139-4140.

Gomez, L.A., A. Alekseev, L. Aleksandrova, P. Brady, A.Terzic. Use of the MTT assay in adult ventricular cardiomyocytes to assess viability: effects of adenosine and potassium on cellular survival.-J. Mol. Cell. Cardiol., 29, 1997, No. 4, 1255.1266

Kiselova-Kaneva, Y., O. Tasinov, D. Vankova, D. Ivanova.Ethanol induced IL-6 and TNFÆ’¿ cytokine and iNOS and COX-2 gene expressionin 3T3-L1 preadipocytes.-Scr. Sci. Med., 44, 2012, No.2, 31.35.

Kumar, A., P. K. Chauhan, V. S. Bhardwaj, R. Kumar, A. Tyagi. In vitro antioxidant and phytochemical investigations of ethanolic extracts of Viola serpens and Morusnigra.-J.Chem. Pharm. Res., 3, 2011, No. 4, 166-171.

Kultur .. Medicinal plants used in K.rklareli Province (Turkey).- J. Ethnopharmacol., 111, 2007, 341-346.

Landzhev, I. In Encyclopedia of Medicinal Plants in Bulgaria. Sofia, Trud Publ., 2010, 372-374 (in Bulgarian).

Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity.-J. Immunol. Methods.,65, 1983, 55.63.

Novacovic, M., I. Vuckovic, P. Janackovic, M. Sokolovic, A. Filipovic, V.Tesevic, S. Milosavljevic. Chemical composition, antibacterial and antifungal activity of the essential oils of Cotinus coggygria from Segbia.- J. Serbian Chem. Soc.,72, 2007, No.11, 1045-1051.

Pasheva, `M., M. Nashar, D. Pavlov, S. Slavova, D. Ivanov, D. Ivanova. Antioxidant capacity of different woods traditionally used for coloring hard alcoholic beverages in Bulgaria.-J. Sci. Technol., 3, 2013, No.1, 123-127.

Pavlov, D., Eftimov, M., Tzaneva, M., Ivanova, D., Nashar, M., Kobakova, I., Valcheva-Kuzmanova, S. 2013. Gastroprotective effect of ethanol infusion from Cotinus coggygria wood in rats. -Scr. Sci. Med., 45 (suppl. 5): 19-24.

Venkatesh, K. R., S. Chauhan. Mulberry: Life enhancer.- J. Med. Plants Res., 2, 2008, No. 10, 271-278.




DOI: http://dx.doi.org/10.14748/ssm.v45i1.993

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About The Authors

Milena Pasheva
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics

Danail Pavlov
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

Oskan Tasinov
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

Milka Nashar
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

Yoana Kiselova-Kaneva
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

Diana Ivanova
Medical University of Varna
Bulgaria

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Medical University of Varna

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