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Microleakage in composite and ceramic restorations—a review of staining protocols

Sabina Keremedchieva, Stefan Peev


Introduction: Various kinds of dye infiltration protocols for the inspection of microleakage in composite and ceramic restorations have been described in the literature.

Aim: The aim of this article is to briefly present the different staining methods used to inspect microleakage in composite and ceramic restorations.

Materials and Methods: This review includes articles searched without date restriction in the Medline/PubMed database. A variety of keywords and their combinations were used: “microleakage”, “staining methods”, “ceramic restorations”, “fuchsine”, “methylene blue”, “silver nitrate”, “composite restorations”, “dye infiltration”.

Results: The review was based upon 71 references. The literature provided information regarding the different staining protocols used for the inspection of microleakage in composite and ceramic restorations.

Conclusion: Numerous types of protocols have been reported. The most common staining agents used for research purposes are fuchsine, methylene blue, and silver nitrate. In order to make the results between different experimental studies comparable, it will be beneficial to have standardized methodologies.


microleakage; direct restorations; indirect restorations; staining methods; fuchsine; methylene blue; silver nitrate

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Cox CF. Microleakage related to restorative procedures. Proc Finn Dent Soc. 1992;88 Suppl 1:83-93.

Rathi SD, Nikhade P, Chandak M, Motwani N, Rathi C, Chandak M. Microleakage in composite resin restoration-a review article. J Evol Med Dent Sci. 2020;9(12):1006-11. doi:10.14260/jemds/2020/216.

Roulet JF. Marginal integrity: clinical significance. J Dent. 1994;22 Suppl 1:S9-12. doi: 10.1016/0300-5712(94)90164-3.

Ferracane JL. Resin composite--state of the art. Dent Mater. 2011;27(1):29-38. doi: 10.1016/

Bajabaa S, Balbaid S, Taleb M, Islam L, Elharazeen S, Alagha E. Microleakage evaluation in class v cavities restored with five different resin composites: in vitro dye leakage study. Clin Cosmet Investig Dent. 2021;13:405-11. doi: 10.2147/CCIDE.S331426.

Cox CF. Microlekkage: evaluatie en behandeling [Microleakage: evaluation and treatment]. Ned Tijdschr Tandheelkd. 1990;97(3):98-100.

Fabianelli A, Pollington S, Davidson CL, Cagidiaco MC, Goracci C. The relevance of microleakage studies. Int Dent SA. 2007; 9(3):64-74.

Majeed A, Osman YI, Al-Omari T. Microleakage of four composite resin systems in class II restorations. SADJ. 2009;64(10):484-8.

Pashley DH. Clinical considerations of microleakage. J Endod. 1990;16(2):70-7. doi: 10.1016/S0099-2399(06)81567-0.

Arisu HD, Uçtasli MB, Eligüzeloglu E, Ozcan S, Omürlü H. The effect of occlusal loading on the microleakage of class V restorations. Oper Dent. 2008;33(2):135-41. doi: 10.2341/07-49.

Pashley EL, Comer RW, Simpson MD, Horner JA, Pashley DH, Caughman WF. Dentin permeability: sealing the dentin in crown preparations. Oper Dent. 1992;17(1):13–20.

Helvey GA. Adhesive dentistry: the development of immediate dentin sealing/selective etching bonding technique. Compend Contin Educ Dent. 2011;32(9):22,24–32, 34–5; quiz 36, 38.

Magne P, Kim TH, Cascione D, Donovan TE. Immediate dentin sealing improves bond strength of indirect restorations. J Prosthet Dent. 2005;94(6):511–9. doi:10.1016/j.prosdent.2005.10.010

Samartzi TK, Papalexopoulos D, Sarafianou A, Kourtis S. Immediate dentin sealing: a literature review. Clin Cosmet Investig Dent. 2021;13:233-256. doi: 10.2147/CCIDE.S307939.

Gladys S, Van Meerbeek B, Lambrechts P, Vanherle G. Microleakage of adhesive restorative materials. Am J Dent. 2001;14(3):170-6.

Taylor MJ, Lynch E. Microleakage. J Dent. 1992;20(1):3-10. doi: 10.1016/0300-5712(92)90002-t.

Shaikh A, Hegde V, Shanmugasundaram S. A new approach to microleakage assessement: review. Int J Curr Adv Res. 2017;6(5):3635-8.

Raskin A, D'Hoore W, Gonthier S, Degrange M, Déjou J. Reliability of in vitro microleakage tests: a literature review. J Adhes Dent. 2001;3(4):295-308.

Kidd EA. Microleakage: a review. J Dent. 1976;4(5):199-206. doi: 10.1016/0300-5712(76)90048-8.

Alani AH, Toh CG. Detection of microleakage around dental restorations: a review. Oper Dent. 1997;22(4):173–85.

Heintze S, Forjanic M, Cavalleri A. Microleakage of Class II restorations with different tracers-comparison with SeM quantitative analysis. J Adhes Dent. 2008;10(4):259–67.

Wu W, Cobb EN. A silver staining technique for investigating wear of restorative dental composites. J Biomed Mater Res. 1981;15(3):343–8.

Wu W, Cobb EN, Dermann K, Rupp NW. Detecting margin leakage of dental composite restorations. J Biomed Mater Res. 1983;17(1):37–43.

Rodrigues SA, Jr, Ferracane JL, Della Bona A. Flexural strength and Weibull analysis of a microhybrid and a nanofill composite evaluated by 3- and 4-point bending tests. Dent Mater. 2008;24:426–31.

Peterson EA 2nd, Phillips RW, Swartz ML.. A comparison of the physical properties of four restorative resins. J Am Dent Assoc. 1966;73(6):1324–36.

Crabtree MG, Atkinson HG. A preliminary report on the solubility of decalcified dentine in water. Austral J Dentistry. 1955;55:340-2.

Palmer DS, Barco MT, Billy EJ. Temperature extremes produced orally by hot and cold liquids. J Prosthet Dent. 1992;67(3):325-7. doi: 10.1016/0022-3913(92)90239-7.

Eliasson ST, Dahl JE. Effect of thermal cycling on temperature changes and bond strength in different test specimens. Biomater Investig Dent. 2020;7(1):16-24. doi: 10.1080/26415275.2019.1709470.

Plant CG, Jones DW, Darvell BW. The heat evolved and temperatures attained during setting of restorative materials. Br Dent J. 1974;137(6):233-8. doi: 10.1038/sj.bdj.4803293.

Nelsen RJ, Wolcott RB, Paffenbarger GC. Fluid exchange at the margins of dental restorations. J Am Dent Assoc. 1952;44(3):288-95. doi: 10.1016/s0002-8177(52)43006-8.

Campos PE, Barceleiro Mde O, Sampaio-Filho HR, Martins LR. Evaluation of the cervical integrity during occlusal loading of Class II restorations. Oper Dent. 2008;33(1):59-64. doi: 10.2341/07-35.

Aggarwal V, Logani A, Jain V, Shah N. Effect of cyclic loading on marginal adaptation and bond strength in direct vs. indirect class II MO composite restorations. Oper Dent. 2008;33(5):587-92. doi: 10.2341/07-152.

Pongprueksa P, Kuphasuk W, Senawongse P. Effect of elastic cavity wall and occlusal loading on microleakage and dentin bond strength. Oper Dent. 2007;32(5):466-75. doi: 10.2341/06-132.

Khvostenko D, Salehi S, Naleway SE, Hilton TJ, Ferracane JL, Mitchell JC, et al. Cyclic mechanical loading promotes bacterial penetration along composite restoration marginal gaps. Dent Mater. 2015;31(6):702-10. doi: 10.1016/

Cooksey C, Dronsfield A. Fuchsine or magenta: the second most famous aniline dye. A short memoir on the 150th anniversary of the first commercial production of this well known dye. Biotech Histochem. 2009;84(4):179-83. doi: 10.1080/10520290903081401.

Ng CH, Ohlin CA, Winther-Jensen B. Characterisation of a series of triarylmethane dyes as light harvesters for photo-electrochemical systems. Dyes Pigm. 2015;115:96-101. doi:10.1016/j.dyepig.2014.12.016.

Kenyon BJ, Frederickson D, Hagge MS. Gingival seal of deep Class II direct and indirect composite restorations. Am J Dent. 2007;20(1):3-6.

Uludag B, Yucedag E, Sahin V. Microleakage of inlay ceramic systems luted with self-adhesive resin cements. J Adhes Dent. 2014;16(6):523-9. doi: 10.3290/j.jad.a32811.

Ovul K, Arzu TM, Ahmet S, Lippo Vj L, Pekka K V. Marginal adaptation and microleakeage of directly and indirectly made fiber reinforced composite inlays. Open Dent J. 2011;5:33-8. doi: 10.2174/1874210601105010033.

Celik EU, Kumbaraci N, Cal E, Turkun M. Influence of two desensitizer agents on the microleakage of adhesively luted ceramic inlays. Eur J Dent. 2011;5(1):77-83.

Hahn P, Schaller HG, Müllner U, Hellwig E. Marginal leakage in class II-restorations after use of ceramic-inserts luted with different materials. J Oral Rehabil. 1998;25(8):567-74. doi: 10.1046/j.1365-2842.1998.00281.x.

Nassaj AE, Ghadimi S, Seraj B, Chiniforush N. Effect of photodynamic therapy on microleakage of class V composite restorations in primary teeth. Photodiagnosis Photodyn Ther. 2020;32:101964. doi: 10.1016/j.pdpdt.2020.101964.

Duquia Rde C, Osinaga PW, Demarco FF, de V Habekost L, Conceição EN. Cervical microleakage in MOD restorations: in vitro comparison of indirect and direct composite. Oper Dent. 2006;31(6):682-7. doi: 10.2341/05-132.

Calabrez-Filho S, Calabrez VC, Reston EG, de Andrade MF, Borges LH. Influence of the internal conditioning of indirect restorations of resin composite in relation to microleakage using LEDs and QTH units. Oper Dent. 2009;34(3):293-8. doi: 10.2341/08-83.

Ziskind D, Elbaz B, Hirschfeld Z, Rosen L. Amalgam alternatives-microleakage evaluation of clinical procedures. Part II: direct/indirect composite inlay systems. J Oral Rehabil. 1998;25(7):502-6. doi: 10.1046/j.1365-2842.1998.00272.x.

Rosentritt M, Behr M, Lang R, Handel G. Influence of cement type on the marginal adaptation of all-ceramic MOD inlays. Dent Mater. 2004;20(5):463-9. doi: 10.1016/

Schmalz G, Federlin M, Reich E. Effect of dimension of luting space and luting composite on marginal adaptation of a class II ceramic inlay. J Prosthet Dent. 1995 Apr;73(4):392-9. doi: 10.1016/s0022-3913(05)80337-3. PMID: 7783020.

Ozturk AN, Ozturk B, Aykent F. Microleakage of different cementation techniques in Class V ceramic inlays. J Oral Rehabil. 2004;31(12):1192-6. doi: 10.1111/j.1365-2842.2004.01361.x.

Keshvad A, Hooshmand T, Asefzadeh F, Khalilinejad F, Alihemmati M, Van Noort R. Marginal gap, internal fit, and fracture load of leucite-reinforced ceramic inlays fabricated by CEREC inLab and hot-pressed techniques. J Prosthodont. 2011;20(7):535-40. doi: 10.1111/j.1532-849X.2011.00745.x.

Hasanreĭsoğlu U, Sönmez H, Uçtaşli S, Wilson HJ. Microleakage of direct and indirect inlay/onlay systems. J Oral Rehabil. 1996;23(1):66-71. doi: 10.1111/j.1365-2842.1996.tb00814.x.

Karaağaclioğlu L, Zaimoğlu A, Akören AC. Microleakage of indirect inlays placed on different kinds of glass ionomer cement linings. J Oral Rehabil. 1992;19(5):457-69. doi: 10.1111/j.1365-2842.1992.tb01110.x.

Thordrup M, Isidor F, Hörsted-Bindslev P. Comparison of marginal fit and microleakage of ceramic and composite inlays: an in vitro study. J Dent. 1994;22(3):147-53. doi: 10.1016/0300-5712(94)90198-8.

Tucker D, Lu Y, Zhang Q. From mitochondrial function to neuroprotection-an emerging role for methylene blue. Mol Neurobiol. 2018;55(6):5137-5153. doi: 10.1007/s12035-017-0712-2.

Xue H, Thaivalappil A, Cao K. The potentials of methylene blue as an anti-aging drug. Cells. 2021;10(12):3379. doi: 10.3390/cells10123379.

Cwalinski T, Polom W, Marano L, Roviello G, D'Angelo A, Cwalina N, et al. Methylene Blue-Current Knowledge, Fluorescent Properties, and Its Future Use. J Clin Med. 2020;9(11):3538. doi: 10.3390/jcm9113538.

Najet AM, Sawsan K, Saida Z, Kamel BS. In vitro comparison of Biodentine and Riva LC interfaces with cervical dentin and Filtek Z350 in posterior class II open sandwich restorations. SRM J Res Dent Sci. 2021;11:178-84.

Gerdolle DA, Mortier E, Loos-Ayav C, Jacquot B, Panighi MM. In vitro evaluation of microleakage of indirect composite inlays cemented with four luting agents. J Prosthet Dent. 2005;93(6):563-70. doi: 10.1016/j.prosdent.2005.04.004.

Shafiei F, Doozandeh M, Alavi AA. Effect of resin coating and chlorhexidine on the microleakage of two resin cements after storage. J Prosthodont. 2011;20(2):106-12. doi: 10.1111/j.1532-849X.2010.00670.x.

Santos ERD, Busato AL. Microleakage in posterior teeth with different materials and different types of cavities. Indian J Dent Res. 2019;30(5):783-787. doi: 10.4103/ijdr.IJDR_113_15.

Peixoto RT, Poletto LT, Lanza MD, Buono VT. The influence of occlusal finish line configuration on microleakage of indirect composite inlays. J Adhes Dent. 2002;4(2):145-50.

Mota CS, Demarco FF, Camacho GB, Powers JM. Microleakage in ceramic inlays luted with different resin cements. J Adhes Dent. 2003;5(1):63-70.

Deliperi S, Bardwell DN, Wegley C. Restoration interface microleakage using one total-etch and three self-etch adhesives. Oper Dent. 2007;32(2):179-84. doi: 10.2341/06-54.

Yeolekar TS, Chowdhary NR, Mukunda KS, Kiran NK. Evaluation of microleakage and marginal ridge fracture resistance of primary molars restored with three restorative materials: a comparative in vitro study. Int J Clin Pediatr Dent. 2015;8(2):108-13. doi: 10.5005/jp-journals-10005-1294.

Gao SS, Zhao IS, Duffin S, Duangthip D, Lo ECM, Chu CH. Revitalising silver nitrate for caries management. Int J Environ Res Public Health. 2018;15(1):80. doi: 10.3390/ijerph15010080.

Chevallet M, Luche S, Rabilloud T. Silver staining of proteins in polyacrylamide gels. Nat Protoc. 2006;1(4):1852-8. doi: 10.1038/nprot.2006.288.

Sinche-Ccahuana I, Ladera-Castañeda M, Paucar-Rodríguez E, Aliaga-Mariñas A, Dapello-Zevallos G, Cervantes-Ganoza L, et al. Microleakage in indirect onlay restorations cemented with three different types of adhesives: An in vitro study. J Clin Exp Dent. 2023;15(8):e641-8. doi: 10.4317/jced.60725.

Fruits TJ, Knapp JA, Khajotia SS. Microleakage in the proximal walls of direct and indirect posterior resin slot restorations. Oper Dent. 2006;31(6):719-27. doi: 10.2341/05-148.

Hasegawa EA, Boyer DB, Chan DC. Microleakage of indirect composite inlays. Dent Mater. 1989;5(6):388-91. doi: 10.1016/0109-5641(89)90106-1.

Romão W Jr, Miranda WG Jr, Cesar PF, Braga RR. Correlation between microleakage and cement thickness in three Class II inlay ceramic systems. Oper Dent. 2004;29(2):212-8.

Naumova EA, Schiml F, Arnold WH, Piwowarczyk A. Marginal quality of ceramic inlays after three different instrumental cavity preparation methods of the proximal boxes. Clin Oral Investig. 2019;23(2):793-803. doi: 10.1007/s00784-018-2492-0.

Bauer JR, Reis A, Loguercio AD, Barroso LP, Grande RH. Effects of aging methods on microleakage of an adhesive system used as a sealant on contaminated surfaces. J Appl Oral Sci. 2005;13(4):377-81.



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About The Authors

Sabina Keremedchieva
Medical University of Varna

Department of Periodontology and Dental Implantology, Faculty of Dental Medicine

Stefan Peev
Medical University of Varna

Department of Periodontology and Dental Implantology, Faculty of Dental Medicine

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