Scientific Online Resource System

Scripta Scientifica Pharmaceutica

INVESTIGATING THE EFFECT OF FISH INTAKE ON METABOLIC HEALTH USING DRIED BLOOD SPOT METHODOLOGY

Khairun Sumali, Sharon Georgina, Sam Philip, Nigel Hoggard, Baukje de Roos

Abstract

Systematic review of data has shown that the relationship between fish consumption and a decreased risk of type 2 diabetes mellitus (T2DM) is mostly evident in the Asian population. Remarkably, tissue levels of omega-3 fatty acids are lower among Asian populations with T2DM. This finding is important especially for South Asian people as they are known to have a greater risk of diabetes compared to other ethnic groups. We hypothesise that fish consumption beneficially modulates omega-3 status and glycaemic control in South Asian people with T2DM living in the UK, and this response is modulated by a single nucleotide polymorphism (TCF7L2 rs7903146). The biomarkers will be assessed using dried blood spot (DBS) technology in replace of venepuncture, allowing the subjects to complete the intervention study in a home setting. DBS sampling requires minimal training, reduces transport burden, and keeps the analytes stable. Although micro-sampling reduced the sensitivity and specificity of assays and limited the use of DBS for many years, advanced analytical techniques have quickly overcome many problems. With this study we are hoping to show that combined with remote sampling, DBS technology offers a feasible alternative in epidemiological studies. We optimised and validated the measurement of the HbA1c, the omega-3 index and triglycerides in DBS, yielding recoveries of >98%, 71-90% and >90%, respectively, compared to whole blood. Furthermore, the role of platelets in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)-mediated cardio-protection will be investigated by assessing the effects of EPA and DHA on platelet activation in healthy versus diabetic European and Asian subjects (2x2 design), using an ex vivo human platelet model. We hypothesise that the effective concentrations of EPA and DHA to inhibit platelet activation may be higher for diabetic compared with non-diabetic subjects as their platelets are in a more pro-coagulant state.

Acknowledgements: Ministry of Education, Malaysia


Keywords

omega 3, PUFA, fish, T2DM, dried blood spot




DOI: http://dx.doi.org/10.14748/ssp.v4i1.3977

Refbacks

Article Tools
Email this article (Login required)
About The Authors

Khairun Sumali

Sharon Georgina

Sam Philip

Nigel Hoggard

Baukje de Roos

Font Size


|