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Changes in interleukin 6 and monocyte chemoattractant protein-1 expression levels in 3t3-l1 cells after exposure to agrimonia eupatoria l. extract and subsequent oxidative stimulation with tert-buthyl hydroperoxide reveal possible adaptogenic potential

Yoana Kiselova, Deyana Vankova, Oskan Tasinov, Inna Feklicheva, Irina Potoroko, Diana Ivanova

Abstract

INTRODUCTION: Synthesis and secretion of many inflammatory factors increase with oxidative stimu- lation and the activity of many transcription factors that regulate synthesis of inflammatory cytokines is in- fluenced by the redox condition in the  cell.

AIM: The aim of the current study was to investigate the effects of aqueous-alcoholic agrimony extract on interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) gene expression levels in cultured preadipocytes under induced oxidative stress  conditions.

MATERIALS AND METHODS: To determine the possible adaptogenic potential of agrimony extract treatment on gene  expression,  preadipocytes were  incubated  in a  medium containing different  amounts  of agrimony extract. Tert-butyl hydroperoxide was used to provoke oxidative response in treated cells. Ex- pression of selected genes was measured using two step quantitative PCR. Results were analyzed using the 2-ΔΔCt   method.

RESULTS: Incubation of preadypocytes with 2.5% agrimony extract resulted in a significant decrease in mRNA levels of MCP-1. Significant increase in IL-6 transcription levels was detected in the cells incubated with 1.25% and 2.5% agrimony extract. Pre-incubation of the cells with agrimony extract prevented subse- quent oxidative induced stimulation of MCP-1 gene expression. Oxidative provocation appeared to decrease the stimulatory activity of agrimony on IL-6 gene  expression.


Keywords

Agrimonia eupatoria; gene expression; MCP-1; IL-6; 3T3-L1; adaptogenic

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DOI: http://dx.doi.org/10.14748/ssp.v4i2.4337

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About The Authors

Yoana Kiselova

Deyana Vankova

Oskan Tasinov

Inna Feklicheva

Irina Potoroko

Diana Ivanova

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