Introduction: Herbal medicinal products are widely used for prevention and treatment of many diseases.
Aim: The aim of our work was to study the parameters of grape leaves extraction on a laboratory extractor Timatic Micro.
Materials and Methods: Towards achieving the goal, the tasks of determining the following factors influencing the grape leaves extraction were set: the degree of grinding and extraction time.
Timatic Micro extractor was used for extraction. This contemporary technology is based on a double action of pressure – reduction of the pressure and percolation of biological material that intensifies extraction process. The extractant used was 40% ethyl alcohol as it provides maximum extraction of flavonoids and tannins. The extractor was set with standard parameters: compression - 2 minutes, decompression - 2 minutes.
Results and Discussion: The degree of raw material depletion in the main biologically active substances of common grape leaves (tannins and flavonoids) significantly increases with decreasing the size of crushed grape leaves, and is the highest in the fraction less than 1 mm and is 69.93 %, 44.13% and 78.48% for extractives, tannins, and flavonoids, respectively (extraction time - 2 h). With increasing the time of extraction up to 4 h the yield of extractible substances increases by an average of 3%, but for 2 hours quite intensive extraction occurs, which gives high rates of raw material depletion.
For obtaining the extract in the laboratory extractor Timatic, we have chosen as raw material a fraction of grape leaves of less than 1 mm and extraction time of 2 hours.
Conclusion: The presented method has proven its reliability and efficiency in the extraction of substances in question.
Reference documents of Ukrainian Ministry of healthcare. Available from: http://mozdocs.kiev.ua/
Omirbaeyeva AE. Study of Carduus crispus dense extracts anti-inflamatory activity. Asian J Sci Educ Res. 2015; 1(17):1004-9.
Alimova US. The study of the antimicrobial activity of CO2 extracts of Plantago major and Acopus calamus. World J Pharm Sci. 2015; 3(5):826-9.
Omirbaeyeva AE. The study of biological active substances of thistle curled (Carduus crispus L.). Int J Pharmacogn Phytochem Res. 2016;8(2):297-9.
Gladukh YeV, Seguy AM. The study of pharmacotechnological parameters of burdock (Arctium lappa) leaves. J Chem Pharm Res. 2016;8(1):260-4.
Gulmurodov IS, Gladukh YeV. The study of rheological properties of the fatty ointment base for topical treatment of colds. J Chem Pharm Res. 2015;7(10):788-96.
Makhsudov K, Stepanenko SV, Gladukh YeV. Pharmaceutical development of ointment for the first phase of wound healing with chamomile extract content. Be in progress 2: International conference of pharmacy students, Lublin, 21st–25th April, 2016. Lublin, 2016. p. 13.
Yudina YuV, Shmyrova YuV, Stepanenko SV, Gladukh IeV, Chyieshov VI, Sichkar AA, et al. Industrial drug technology: tutorial for laboratory classes for students of speciality "Pharmacy". Kharkiv: Original; 2012.
Kumarova AK, Gladukh YeV, Kukhtenko GP. Research of the extraction process in the preparation of camel thorn dense extract. Topical issues of new drugs development: abstracts оf international scientific and practical conference of young scientists and student, April 23, 2015. Kharkiv; 2015. p. 205-206.
Rakhmonov AU, Kukhtenko GP, Gladukh YeV. Research of the liquid nettle leaves extract obtaining by percolation method. Topical issues of new drugs development: abstracts оf international scientific and practical conference of young scientists and student, April 23, 2015. Kharkiv; 2015. p. 165.
Kha DA, Kukhtenko ОS, Gladukh YeV. Development of technology for producing compound thick extract obtained by common extraction of thyme, yarrow, chamomile and eucalyptus. Topical issues of new drugs development: abstracts оf XXIII international scientific and practical conference of young scientists and student, April 21, 2016. Kharkiv; 2016 (1). p. 267.
Iudina Yu. The study of the calendula flowers extraction process. Visnyk pharmacii. 2015; 3(83):28-31.
Vinograd kulturnij. Available from: http://www.pharmencyclopedia.com.ua/article/1711/vinograd-kulturnij
Dresch R, Dresch M, Guerreiro A, Biegelmeyer R, Holzschuh M, Rambo D, Henriques A. Phenolic compounds from the leaves of Vitis labrusca and Vitis vinifera L. as a source of waste byproducts: development and validation of LC method and antichemotactic activity. Food Anal Methods. 2014; 7:527–39.
Bondarenko AS, Bevz NY, Gladukh IeV, Soldatov DP. Quantification of the main active ingredients of plant extracts to establish optimal conditions for extraction. J Chem Pharm Res. 2015;7(2):618-25
Timatic Micro. Available from: http://www.timatic.it/index.php/en/products/extractors-of-active/timatic-micro
Email this article (Login required) 
