Introduction: UV spectrophotometry is the simplest method of quantitative analysis of active pharmaceutical ingredients. It is widely used in developing active pharmaceutical ingredients, quantifying impurities, dissolution testing, and quantifying nucleic acids and proteins in
pharmaceutical development. However, there is no convenient library of UV spectra of pharmaceutical ingredients in pure distilled water, and it is sometimes hard to find the necessary information in the literature.
Aim: This study aims to create a dataset of UV spectra of 27 common pharmaceutical ingredients in aqueous or (for those insoluble in water) ethanolic solutions and to review the analytical methods of determination of these ingredients based on these spectral data that were already developed.
Materials and Methods: The aqueous (or ethanolic for those insoluble in water) solutions of 27 active pharmaceutical ingredients were prepared, and their UV spectra were recorded.
Results and Conclusion: UV spectra of aqueous solutions of amlodipine besylate, articaine hydrochloride, bendazole hydrochloride, betaxolol hydrochloride, carbamazepine, citicoline sodium, chloropyramine, clopidogrel bisulfate, dexketoprofen trometamol, drotaverine hydrochloride, 2-ethyl-6-methyl-3-hydroxypyridine succinate, ketorolac trometamol, loperamide hydrochloride, menadione sodium bisulfite, metamizole sodium, metoclopramide hydrochloride, metoprolol tartrate, moxifloxacin hydrochloride, nicotinic acid (niacin), norepinephrine hydrochloride (noradrenaline hydrochloride), paracetamol (acetaminophen), and valacyclovir, and ethanolic solutions of carvedilol, haloperidol, indapamide, ketoprofen, and spironolactone are presented. The wavelengths of absorbance maxima for each compound are given. The methods of UV-spectrophotometric determination of these active pharmaceutical ingredients available in literature are reviewed.
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