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Cellular proliferation in the cortex of postischemic cerebellum by primates

Veselina Mihaleva, Vanya Goranova, Meglena Angelova, Stoyan Pavlov, Tetsumori Yamashima, Anton Tonchev

Abstract

Tissue and cellular mechanisms of damage and restoration аfter brain ischemia have fundamental as well as practical significance. The goal of the present study was to determine changes in cellular proliferation by bromodeoxyuridin (BrdU), indicator of DNA synthesis, in the cerebellum of newborn, one year old and adult Japanese monkeys (Macaca fuscata) after global brain ischemia. According to the age, BrdU paradigm (2 days х 250 mg/kg i.v. or 5 days х 100 mg/kg) and survival period after ischemia (4, 9 and 15 days correspondingly) the animals were distributed in several control and experimental groups. We applied immunohistochemical staining with peroxidase on frozen sections from cerebella of the monkeys examined to demonstrate the proliferative marker BrdU. Сomparison of thе аmount of BrdU+ cells in cerebellar cortex between non-ischemic control groups of different age showed a significant age dependent decline in the proliferative processes acheaving a relatively low supporting level. Comparison between groups of adult monkeys, subjected to ischemia with different duration, indicated that it is an important factor which induced cellular proliferation in the cerebellum but this effect declined with the time after ischemia. A forthcoming phenotyping of BrdU+ cells will differentiate between types of newborn cells and their relationship in the postischemic cerebellar cortex. The data acquired may expand and enlarge the understanding about restorative processes after ischemic injury to the cerebellum in the primates examined.

Keywords

ischemia; proliferation; BrdU; cerebellàr cortex; primates

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DOI: http://dx.doi.org/10.14748/vmf.v4i1.1221

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