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Varna Medical Forum

Toxocara seroprevalence among risk group for zoonotic distribution and healthy adults

Tatyana Cvetkova, Kalina Stoyanova

Abstract

Introduction: Toxocarosis is a chronic zoonosis that occurs in a systemic, ocular or covert form. The infection is typical for children and presumably rare in adults.
Aim: The aim of the present study is to compare the seroprevalence of anti-Toxocara antibodies between a group with elevated risk for zoonotic distribution and healthy controls. Materials and Methods: The subject of the study is an actively selected group of 60 persons with increased occupational and/or behavioral risk for transmission of zoonoses (veterinarians, pet owners, etc.). The data concerning risk factors and clinical symptoms is gathered through individual questionnaires. The seroprevalence of anti-Toxocara antibodies was determined by enzyme-linked immunosorbent assay (ELISA) and all positive results were verified with Western blot. The results were compared against a control group of randomly chosen 60 healthy adult blood donors.
Results and Discussion: The overall seroprevalence in the risk group is 8.33%, with higher rate in males - 15.8% than females - 4.8%. Unexpectedly higher levels (15.0%) were observed in the control group (23.3% males, 6.67% females). These results reveal previously undetected hidden morbidity among the adult population of northeastern Bulgaria and put toxocarosis at the head of distribution among parasitic zoonoses in the region. The lower seroprevalence status of the risk group can be attributed to the increased awareness and active prophylaxis of the zoonotic diseases by the veterinarians and pet owners.


Keywords

toxocarosis; seroprevalence; ELISA; Western blot

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References

Георгиева Д., Койнарски В., Перелезов П., Иванов А. Паразитологично изследване на проби пръст и пясък от различни райони на град Стара Загора. Стара Загора:, 1998. 34.

Кънева Е, Райнова И, Минева И, Харизанов Р, Кафтанджиев И. Клинични симптоми на токсокароза при изследвани пациенти за периода 2004-2013г., Medicine. 2014; 1 (4) : 216–221.

Borg OA, Woodruff AW. Prevalence of infective ova of Toxocara species in public places.British medical journal. 1973; 5890 (4). 470.

Despommier D. Toxocariasis: Clinical Aspects, Epidemiology, Medical Ecology, and Molecular Aspects.Clinical Microbiology Reviews. 2003; 2(16).265–272.

Dickson D. Toxocariasis: clinical aspects, epidemiology, medical ecology, and molecular aspects. Clin Microbiol Rev. 2003;16(2): 265–272.

Deutz A, Fuchs K, Auer H, Kerbl U, Aspöck H, Köfer J. Toxocara-infestations in Austria: a study on the risk of infection of farmers, slaughterhouse staff, hunters and veterinarians. Parasitology Research. 2005; 5 (97): 390–394.

Fu CJ, Kao CY, Lee YL, Liao CW, Chen PC, Chuang TW(et al.). Seroprevalence and Associated Risk Factors of Toxocariasis among College Students in Taipei City, Taiwan.I ranian Journal of Parasitology. 2015; 3 (10): 482–489.

Gabrielli S, Tasić-Otašević S, Ignjatović A, Fraulo M, Trenkić-Božinović M, Momčilović S. Seroprevalence and Risk Factors for Toxocara canis Infection in Serbia During 2015.Foodborne Pathogens and Disease. 2016; 1 (14): 43–49.

Gamboa M.I. Effects of temperature and humidity on the development of eggs of Toxocara canis under laboratory conditions. Journal of Helminthology. 2005; 4(79): 327–331.

Glickman L.T., Schantz P.M. Epidemiology and pathogenesis of zoonotic toxocariasis. Epidemiologic Reviews. 1981; (3): 230–250.

Goto H, Mochizuki M, Yamaki K, Kotake S, Usui M, Ohno S. Epidemiological Survey of Intraocular Inflammation in Japan. Jap. Journ. of Ophthalm. 2007;1 (51):41–44.

Gueglio B, Gentile L, Nguyen JM, Achard J, Chabasse D, Marjolet M. Epidemiologic approach to human toxocariasis in western France. Parasitol. Research. 1994; 6 (80):531–536.

Havasiová K, Dubinský P, Stefancíková A. A seroepidemiological study of human Toxocara infection in the Slovak Republic. Journal of Helminthology. 1993; 4 (67): 291–296.

Huminer D, Symon K, Groskopf I, Pietrushka D, Kremer I, Schantz PM, Pitlik SD. Seroepidemiologic study of toxocariasis and strongyloidiasis in institutionalized mentally retarded adults. The Am. Journal of Trop. Medicine and Hygiene. 1992; 3 (46): 278–281.

Jeanneret JP. Epidémiologie de la toxocarose dans la région jurassienne 1991.

Jeleva R, Radeva I, Dicov I, Vatchkov P, Duchovnikova T, Ratchev P at al. First case of toxocaral meningitis in Bulgaria. Mediterranean Journal of Infectious and Parasitic Diseases. 1998; (13): 49–52.

Kanafani ZA, Skoury A, Araj GF, El-Khoury M, Sawaya RA, Atweh SF. Seroprevalence of toxocariasis in Lebanon: a pilot study. Parasitology. 2006; 5 (132): 635–639.

Klapec T, Borecka A. Contamination of vegetables, fruits and soil with geohelmints eggs on organic farms in Poland. Annals of Agricultural and Environmental Medicine. 2012; 3 (19): 421-425

Macpherson CN. Human behaviour and the epidemiology of parasitic zoonoses. International Journal for Parasitology. 2005;11–12 (35): 1319–1331.

Magnaval JF, Michault A, Calon N, Charlet JP. Epidemiology of human toxocariasis in La Réunion. Transactions of The Royal Society of Tropical Medicine and Hygiene. 1994; 5 (88): 531–533.

Malla N, Aggarwal AK, Mahajan R. A serological study of human toxocariasis in north India. National Medical Journal of India. 2002; 3 (15):145–147.

Manini MP, Marchioro AA, Colli CM, Nishi L, Falavigna-Guilherme AL. Association between contamination of public squares and seropositivity for Toxocara spp. in children.Veterinary Parasitology. 2012;1-2 (188):48-52.

Negri EC, Santarém VA, Rubinsky-Elefant G, Giuffrida R. Anti-Toxocara spp. antibodies in an adult healthy population: serosurvey and risk factors in Southeast Brazil. Asian Pacific Journal of Tropical Biomedicine. 2013; 3 (3): 211-216.

Nicoletti A, Sofia V, Mantella A, Vitale G, Contrafatto D, Sorbello V et al. Epilepsy and toxocariasis: a case-control study in Italy. Epilepsia. 2008; 4 (49):594-599.

Stoyanova. K., Cvetkova T, Pavlov S, Todorov S, Gospodinova M. Prevalence of Intestinal and Non-intestinal Parasitoses in Varna region for 2013-2015 year: data from outpatient parasitological testing. Scripta Scientifica Medica.2016; 48 (2):45-49

Radman NE, Archelli SM, Fonrouge RD, Guardis M, Linzitto OR. Human toxocarosis. Its seroprevalence in the City of La Plata. Memórias do Instituto Oswaldo Cruz. 2000;3 (95):281-285.

Rainova I., Kurdova R. Specifying clinical forms of toxocarosis after testing serologically suspected patients. Problems of Inf. and Paras. Dis. 2007; Vol.35(1):26-27.

Roddie G, Stafford P, Holland C, Wolfe A. Contamination of dog hair with eggs of Toxocara canis. Veterinary parasitology. 2008; 1 (152): 85-93.

29. Safar EH, Ghaffar FM, Saffar SA, Makled KM, Habib KS, Abiad R et al. Incidence of Toxoplasma and Toxocara antibodies among out-patients in the Ophthalmic Research Institute, Egypt. Journ. of the Egyptian Soc. of Parasitology. 1995; 3 (25):839-852.

Smith H, Holland C, Taylor M, Magnaval JF, Schantz P, Maizels R. How common is human toxocariasis? Towards standardizing our knowledge. Trends in Parasitology. 2009; 4 (25): 182-188.

Sozen H, Citil BE, Caylak S, Gokmen A, Kaya S, Demirci M et al. Seroepidemiological Study of Toxocariasis among Volunteers Animal Husbandry Workers and Veterinary in Southern Anatolia in Turkey in 2014. Iranian Journal of Parasitology. 2015; 3 (10): 473-481.

Stensvold CR, Skov J, Møller LN, Jensen PM, Kapel CM, Petersen E. Seroprevalence of Human Toxocariasis in Denmark. Clinical and Vaccine Immunology. 2009; 9 (16):1372-1373.

Torgerson PR, Rosenheim K, Tanner I, Ziadinov I, Grimm F, Brunner M et al. Echinococcosis, Toxocarosis and Toxoplasmosis screening in a rural community in eastern Kazakhstan.Tropical Medicine & International Health. 2009;3 (14): 341-348.

Uhlikova M., Hübner J. Seroprevalence of Toxocara canis infection in Czech Republic. Central european journal of public health. 1998;3 (6):195–198.




DOI: http://dx.doi.org/10.14748/vmf.v6i0.5463

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