Scientific Online Resource System

Varna Medical Forum

Microribonucleic acids in children with Crohn’s disease

Krasimira Koleva, Miglena Georgieva

Abstract

In recent years, several microribonucleic acids (microRNA) have been used as promising non-invasive biomarkers in patients with ulcerative colitis. Quantitative characteristics of the expression of 11 different serum microRNAs were examined by means of real-time polymerase chain reaction in a total of 33 children. There were 22 children, 16 girls and six boys, aged between nine and 17 years with Crohn’s disease and 20 healthy children, 12 girls and eight boys, aged between four and 17 years. There was a higher expression of all the microRNAs in patients than in healthy children. The values of microRNA142-3p and microRNA642-b3 were statistically reliably greater in children with Crohn’s disease than in healthy children (t=2.05; p<0.05 and t=2.00; p<0.05, respectively). Some microRNAs play a diagnostic role in children with Crohn’s disease. An additional systematic research is needed for children and adults with this disease.

Keywords

Crohn’s disease, microribonucleic acids, children

Full Text


References

Altaf-Ul-Amin M, Karim MB, Hu P, Ono N, Kanaya S. Discovery of inflammatory bowel disease-associated miRNAs using a novel bipartite clustering approach. BMC Med Genomics. 2020;13(Suppl 3):10. doi: 10.1186/s12920-020-0660-y.

Béres NJ, Szabó D, Kocsis D, Szűcs D, Kiss Z, Müller KE, et al. Role of altered expression of miR-146a, miR-155, and miR-122 in pediatric patients with inflammatory bowel disease. Inflamm Bowel Dis. 2016;22(2):327-335.

Buonpane C, Ares G, Benyamen B, Yuan C, Hunter CJ. Identification of suitable reference microRNA for qPCR analysis in pediatric inflammatory bowel disease. Physiol Genomics. 2019;51(5):169-175.

Chapman CG, Pekow J. The emerging role of miRNAs in inflammatory bowel disease: a review. Therap Adv Gastroenterol. 2015;8(1):4-22.

Chen Y, Wang C, Liu Y, Tang L, Zheng M, Xu C, et al. MiR-122 targets NOD2 to decrease intestinal epithelial cell injury in Crohn's disease. Biochem Biophys Res Commun. 2013;438(1):133-139.

Keith BP, Barrow JB, Toyonaga T, Kazgan N, O’Connor MH, Shah ND, et al. Colonic epithelial miR-31 associates with the development of Crohn’s phenotypes. JCI Insight. 2018;3(19):e122788.

Malmborg P, Hildebrand H. The emerging global epidemic of paediatric inflammatory bowel disease - causes and consequences. J Intern Med. 2016;279(3):241-258.

Mehta SJ, Lewis A, Nijhuis A, Jeffery R, Biancheri P, Di Sabatino A, et al. Epithelial down-regulation of the miR-200 family in fibrostenosing Crohn’s disease is associated with features of epithelial to mesenchymal transition. J Cell Mol Med. 2018;22(11):5617-5628.

Min M, Peng L, Yang Y, Guo M, Wang W, Sun G. MicroRNA-155 is involved in the pathogenesis of ulcerative colitis by targeting FOXO3a. Inflamm Bowel Dis. 2014;20(4):652-659.

Peck BC, Weiser M, Lee SE, Gipson GR, Iyer VB, Sartor RB, et al. MicroRNAs classify different disease behavior phenotypes of Crohn’s disease and may have prognostic utility. Inflamm Bowel Dis. 2015;21(9):2178-2187.

Roberts SE, Thorne K, Thapar N, Broekaert I, Benninga MA, Dolinsek J, et al. A systematic review and meta analysis of paediatric inflammatory bowel disease incidence and prevalence across Europe. J Crohns Colitis. 2020 Feb 28. doi: 10.1093/ecco-jcc/jjaa037.

Szűcs D, Béres NJ, Rokonay R, Boros K, Borka K, Kiss Z, et al. Increased duodenal expression of miR-146a and -155 in pediatric Crohn's disease. World J Gastroenterol. 2016;22(26):6027-6035.

Tang WJ, Peng KY, Tang ZF, Wang YH, Xue AJ, Huang Y. MicroRNA-15a - cell division cycle 42 signaling pathway in pathogenesis of pediatric inflammatory bowel disease. World J Gastroenterol. 2018;24(46):5234-5245.

Wang H, Chao K, Ng SC, Bai AH, Yu Q, Yu J, et al. Pro-inflammatory miR-223 mediates the cross-talk between the IL23 pathway and the intestinal barrier in inflammatory bowel disease. Genome Biol. 2016;17:58. doi: 10.1186/s13059-016-0901-8.

Wang H, Zhang S, Yu Q, Yang G, Guo J, Li M, et al. Circulating microRNA223 is a new biomarker for inflammatory bowel disease. Medicine (Baltimore). 2016а;95(5):e2703. doi: 10.1097/MD.0000000000002703.

Xu XM, Zhang HJ. miRNAs as new molecular insights into inflammatory bowel disease: Crucial regulators in autoimmunity and inflammation. World J Gastroenterol. 2016;22(7):2206-2218.

Zahm AM, Thayu M, Hand NJ, Horner A, Leonard MB, Friedman JR. Circulating microRNA is a biomarker of pediatric Crohn disease. J Pediatr Gastroenterol Nutr. 2011;53(1):26-33.

Zahm AM, Hand NJ, Tsoucas DM, Le Guen CL, Baldassano RN, Friedman JR. Rectal microRNAs are perturbed in pediatric inflammatory bowel disease of the colon. J Crohns Colitis. 2014;8(9):1108-1117.




DOI: http://dx.doi.org/10.14748/vmf.v9i2.6661

Refbacks

User Guide

Font Size


|