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Expression Of Micro-Ribonucleic Acid 451 When Used With Chronic Inflammatory Bowel Disease

Antonia Atanassova

Abstract

Introduction:

MicroRNA-451 was first identified more than a decade ago in human pituitary tissue. There is growing evidence of the important role of miR-451 in immune-mediated diseases. Chronic inflammatory bowel disease (IBD) is an example of an immune-mediated disease with unclear pathogenesis and difficult to follow activity due to the diverse clinical picture. Therefore, we aimed to determine the role of miR-451 expression in patients with Crohn's disease (CD) and ulcerative colitis (UC).

Material and Methods:

A total of 35 consecutive patients with UC, 35 patients with CD, and a control group of 30 healthy subjects were included. In all of them, the serum expression of miR-451 was examined by reverse transcriptase—quantitative real-time polymerase chain reaction PCR (RT-qPCR). The results were statistically processed with SPSS v. 20.0 for Windows, using variation, variance, comparative, correlation analysis, and ROC curve analysis.

Results:

Circulating levels of microRNA-451 in patients with IBD are associated with active disease, intestinal localization, vitamin deficiency. Vitamins B12 and D3 and corticosteroid treatment are recommended.

Conclusion:

Due to the limited number of IBD studies, extensive cohort studies are needed to validate the established results.


Keywords

microRNA-451, Crohn's disease, ulcerative colitis, expression

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