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Purification of Enolase from Human Brain Tissue

B. Galunska, D. Zanev, H. Divanyan


Neuron-specific enolase (NSE) and non-neuronal enolase (NNE) were purified using a small number of methods performed in the following order: DEAE-cellulose chromatography, diafiltration, preparative isoelectric focusing and gel-filtration. Pure isoenzymes were isolated in quantities, which would allow us to produce antisera against each form of the enzyme.


Neuron-Specific Enolase; Non-Neuronal Enolase; Purification of Enolase; DEAE-Chromatography; Preparative Isoelectring Focusing; Electrophoresis

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About The Authors

B. Galunska


B. Galunska, Dept, of Preclinical and Clinical Pharmacology and Biochemistry, Prof. P. Stoyanov Medical University of Varna. 55 Marin Drinov St, BG-9002 Varna, BULGARIA
E-mail: pharmacol@asclep.muvar.acadbg

D. Zanev

H. Divanyan


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