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The Role of Vascular Endothelial Growth Factor and Antithrombin III In The Pathogenesis of the Ovarian Hyperstimulation Syndrome

E. Kovachev, I. Kozovski, V. Markova, K. Aleksandrov, S. Cherneva, S. Cvetkova


Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication of controlled ovarian hyperstimulation (COH). Its pathogenesis is not clarified yet. In the recent years a number of studies focused on the vascular endothelial growth factor (VEGF) and antihtrombin III (AT III) indicators. VEGF is homodimeric, heparin-binding glycoprotein, stimulating vascular permeability. Antithrombin III is protease inhibitor of activated clotting factors. This study aimed at examining the VEGF-A165 and AT III indicators with two OHSS patients. Two methods were used for the determination of the indicators of VEGF-A165 and AT III: ELISA for VEGF and chromogenic assay for ATT III. Kits of R/D Systems and American Diagnostica Inc. were used to estimate VEGF and AT III indicators in serum and plasma. There were higher indicators of VEGF-A165 (180pg/ml) and reduction of AT III indicators (48%) in the patient with a severe form of OHSS than in the control group while these indicators were normal in the patient with a moderate form of OHSS. Our results confirmed some published data concerning the importance of VEGF and AT III in the genesis of OHSS. This study should include a larger group of patients in order tofollow-up statistically and authentically the variations of the indicators of both factors and their importance for OHSS.


OHSS; Assisted Reproductive Technology; Ovarian Hyperstimulation Syndrome; VEGF; AT III

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About The Authors

E. Kovachev


E. Kovachev, Dept, of Obstetrics and Gynaecology, Prof. Paraskev Stoyanov Medical University of Varna, BG-9002 Varna, 55 Marin Drinov St., Bulgaria

I. Kozovski


V. Markova


K. Aleksandrov

S. Cherneva


S. Cvetkova

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