RNA isolation from formalin-fixed paraffin embedded tissue has been a target for improvement for many years. The major limitation is the highly degraded RNA, which in turn increases the demand of the highest yields possible during isolation procedures. In this study, we compared the purification efficacy of RNA between the traditional ethanol precipitation and a modified protocol, which includes the use of silica-based spin-columns for RNA purification and recovery. We also modified and assessed the efficacy of a protocol for effective decontamination and regeneration of spin-columns.
Chung J-Y, Braunschweig T, Williams R, Guerrero N, Hoffmann KM, Kwon M, et al. Factors in tissue handling and processing that impact RNA obtained from formalin-fixed, paraffin-embedded tissue. J Histochem Cytochem [Internet]. 2008;56(11):1033–42. Available from: http://www. pubmedcentral.nih.gov/articlerender.fcgi?artid=256 9903&tool=pmcentrez&rendertype=abstract
Gilbert MTP, Haselkorn T, Bunce M, Sanchez JJ, Lucas SB, Jewell LD, et al. The Isolation of Nucleic Acids from Fixed, Paraffin-Embedded Tissues- Which Methods Are Useful When? PLoS One. 2007;2(6). doi: 10.1371/journal.pone.0000537
Doleshal M, Magotra AA, Choudhury B, Can¬non BD, Labourier E, Szafranska AE. Evaluation and validation of total RNA extraction meth¬ods for microRNA expression analyses in formalin-fixed, paraffin-embedded tissues. J Mol Diagn [Internet]. 2008;10(3):203–11. Available from: http://www.sciencedirect.com/science/article/pii/S1525157810601511. doi: 10.2353/ jmoldx.2008.070153
Bohmann K, Hennig G, Rogel U, Poremba C, Mueller BM, Fritz P, et al. RNA extraction from archival formalin-fixed paraffin-embedded tissue: A comparison of manual, semiautomated, and fully automated purification methods. Clin Chem. 2009;55(9):1719–27. doi: 10.1373/ clinchem.2008.122572
Li J, Smyth P, Flavin R, Cahill S, Denning K, Aherne S, et al. Comparison of miRNA expression patterns using total RNA extracted from matched samples of formalin-fixed paraffin-embedded (FFPE) cells and snap frozen cells. BMC Biotechnol. 2007;7:36. doi: 10.1186/1472-6750-7-36
von Ahlfen S, Missel A, Bendrat K, Schlumpberger M. Determinants of RNA quality from FFPE samples. PLoS One. 2007;2(12). doi: 10.1371/journal. pone.0001261
Tan SC, Yiap BC, Tan SC, Yiap BC. DNA, RNA, and protein extraction: the past and the present. J Biomed Biotechnol [Internet]. 2009;2009:574398. Available from: http://www.ncbi.nlm.nih.gov/ pubmed/20011662%5Cnhttp://www.pubmedcen¬tral.nih.gov/articlerender.fcgi?artid=PMC2789530. doi: 10.1155/2009/574398
Lee HY, Park MJ, Kim NY, Sim JE, Yang WI, Shin KJ. Simple and highly effective DNA extraction methods from old skeletal remains using silica columns. Forensic Sci Int Genet. 2010;4(5):275–80. doi: 10.1016/j.fsigen.2009.10.014
Nicosia A, Tagliavia M, Costa S. Regeneration of total RNA purification silica-based columns. Biomed Chromatogr. 2010;24(12):1263–4.
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