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Scripta Scientifica Medica

Effects of kyotorphin on the morphological changes in the somatosensory cortex and meningeal blood vessels of Alzheimer`s disease model rats

B. Landzhov, H. Angelova, A. Iliev, S. Stanchev, L. Malinova, D. Pechlivanova, E. Dzhambazova

Abstract

Alzheimer`s disease (AD) is a neurodegenerative disease associated with a progressive loss of memory, personality changes and deterioration of the intellectual and social functions, which occurs after a prolonged pre-symptom­atic phase. Abnormal deposits of beta-amyloid plaques that build up in the spaces between nerve cells and tangles (twisted fibers) that build up inside cells, are prime suspects in damaging and killing nerve cells. Recently, in the cerebrospinal fluid of AD patients, reduced levels of neuropeptide kyotorphin (KTP), accompanied by increase in p-tau, a marker of neurodegeneration, were found.

The goal of the present work was to study the effects of KTP on the morphological changes in the somatosenso­ry cortex and meningeal blood vessels of AD model rats. We used bilateral intracerebroventricular injection of streptozotocin (STZ) in Wistar rats (300-350 g) as a model of sporadic AD. Animals were randomly divided in three groups: a control group (sham-operated and injected with saline), an AD group (injected with STZ) and an AD+KTP group (injected with STZ and treated with KTP). Neuritic plaques in the somatosensory cortex and blood vessels were confirmed using thioflavin S fluorescent staining and visualized by microscopy at 40x mag­nification. Plaques were quantified using an image analyzer (CUE-2, Olympus America, Center Valley, PA, USA) and recorded as a percentage of the total area. Our results showed that there was a significant increase in the thioflavin-S accumulation in AD and AD+KTP groups compared to controls (p<0.05) and a tendency of a de­crease in the AD+KTP group compared to the AD group. This data showed slight but significant protective effect of intracerebral KTP against AD-induced morphological abnormalities.





DOI: http://dx.doi.org/10.14748/ssm.v49i0.4844
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About The Authors

B. Landzhov
Medical University of Sofia
Bulgaria

H. Angelova
Medical University of Sofia
Bulgaria

A. Iliev
Medical University of Sofia
Bulgaria

S. Stanchev
Medical University of Sofia
Bulgaria

L. Malinova
Medical University of Sofia
Bulgaria

D. Pechlivanova
Medical University of Sofia
Bulgaria

E. Dzhambazova
Medical University of Sofia
Bulgaria

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