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Microelement compounds in healthy long and irregular bones

Maksym Pogorielov, Gennadii Tkach, Oksana Pogorielova, Vladimir Deineka, Vitalii Sikora, Evgenii Gusak, Sergii Danilchenko


PURPOSE: One of the most important functions of the osseous tissue is to deposit macro- and microelements. For that reason, the investigation of the skeleton ion profile is highly important to explain the physiological and reparative osteogenesis. The osseous tissue, compared to blood, is a more stable system and ionconcentration in bone can display the chronic disorders of its metabolism and its distribution around the body. Ion disbalance can affect bone cells and change the structure and function of the skeleton. The long and irregular bones have different metabolic activities and vascularization, so the ion distribution must be different, but information about ion balance is not clear. This determined the purpose of the research, which deals with microelement compounds of tibia, femoral bone and 2nd lumbar vertebra.

MATERIAL AND METHODS: The paper deals with analysis of osseous tissue samples from 20 5-monthold white rats. We measured the levels of Fe, Mg, Zn, Cu, Co, Mn, Ni, Pb and Cd in tibia, femur and 2nd lumbar vertebra. The research results were analyzed according to generally accepted methods of variance analysis with the help of MS Excel software program.

RESULTS AND CONCLUSIONS: The elemental analysis of different bone types shows diversities between tubular and irregular bones` microelement composition. The lumbar vertebrae contain a great amount of essential elements which is related to its remodeling activity and availability of enzymes. Mostly the long bones store toxic elements, such as Ni and Pb. This indicates a lower speed of the remodeling process of compact substance. Thus, elementary bone compounds directly depend on osseous tissue structure.


long bones; irregular bones; trace elements

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About The Authors

Maksym Pogorielov
Sumy State University of Sumy

Gennadii Tkach
Sumy State University of Sumy

Oksana Pogorielova
Sumy State University of Sumy

Vladimir Deineka
Sumy State University of Sumy

Vitalii Sikora
Sumy State University of Sumy

Evgenii Gusak
Institute of Applied Physics of Sumy

Sergii Danilchenko
Institute of Applied Physics of Sumy

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